BBa_K634004 1 BBa_K634004 J23100:exaDE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Cloning of BBa_J23100, BBa_K634002. An active form of BBa_K634002 under the constitutive J23100 promoter. false false _809_ 0 9393 9 Not in stock false N/A false Kenneth O. Xu component2129738 1 BBa_K634002 component2129731 1 BBa_J23100 annotation2129731 1 BBa_J23100 range2129731 1 1 35 annotation2129738 1 BBa_K634002 range2129738 1 44 1584 BBa_K634002 1 BBa_K634002 exaDE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Cloning of k634000 and k634001. The 2 component system that responds to ethanol that activates transcription of pexaA. false false _809_ 0 9393 9 Not in stock false Each part contains a RBS but the part does not contain a promoter. false Kenneth O. Xu component2129709 1 BBa_K634000 component2129712 1 BBa_K634001 annotation2129709 1 BBa_K634000 range2129709 1 1 841 annotation2129712 1 BBa_K634001 range2129712 1 850 1541 BBa_K634001 1 BBa_K634001 exaE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z de novo synthesis original amino acid sequence from pseudomonas aeruginosa. Released HQ 2013 exaE is a transcription factor that is activated by phosphorylation from exaD. It acts upon pexaA. false false _809_ 0 9393 9 In stock false contains no biobrick cutsites false Kenneth O. Xu annotation2129705 1 rbs range2129705 1 1 14 annotation2129706 1 exaE range2129706 1 15 692 BBa_K634000 1 BBa_K634000 exaD 2011-09-11T11:00:00Z 2015-05-08T01:12:57Z De novo synthesis, codon optimized for E. coli. Original amino acid sequence taken from Pseudomonas aeruginosa. ExaD phosphorylates ExaE, which is a transcription factor acting upon PexaA. false false _809_ 0 9290 9 It's complicated false The synthesis included a Shine-Dalgarno-complimentary RBS 5' of the gene. false Eric Walters annotation2127310 1 RBS range2127310 1 9 22 annotation2127311 1 exaD range2127311 1 23 841 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K634001_sequence 1 aggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa BBa_K634004_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagaggtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaatactagagaggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa BBa_K634000_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaa BBa_K634002_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaatactagagaggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z