LOCUS       BBa_K634007             3170 bp    DNA     linear       27-Dec-2024
DEFINITION  Double selection cassette (tagRFP-sacB-kanR)
ACCESSION   BBa_K634007
VERSION     BBa_K634007.1  
FEATURES             Location/Qualifiers
     RBS             1..14
                     /label=rbs
     CDS             15..750
                     /label=tagRFP
     misc_feature    1..750
                     /label=BBa_K634005
     RBS             759..761
                     /label=RBS
     CDS             771..2192
                     /label=Coding sequence
     misc_feature    969..974
                     /label=Removed EcoRI site
     misc_feature    759..2195
                     /label=BBa_K322921
     CDS             2352..3170
                     /label=kanamycin resistance
     misc_feature    2204..3170
                     /label=BBa_P1003
ORIGIN
        1 aggaggaaaa aaaaatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
       61 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
      121 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
      181 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
      241 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
      301 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
      361 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
      421 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
      481 gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc cacctgatct
      541 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
      601 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
      661 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
      721 ttaatggcat ggacgagctg tacaagtaat tactagagga gacatgaacg atgaacatca
      781 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
      841 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt
      901 cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc
      961 aagttcctga gttcgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg
     1021 tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc
     1081 acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt
     1141 tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta
     1201 aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt
     1261 caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg
     1321 gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca
     1381 gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa
     1441 cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata
     1501 cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa
     1561 acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg
     1621 gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac
     1681 gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac
     1741 tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg
     1801 cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc ggatcaaaaa
     1861 tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt
     1921 taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc
     1981 ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg
     2041 tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg
     2101 cgccaagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
     2161 ttgaacaagg acaattaaca gttaacaaat aataatacta gagctgatcc ttcaactcag
     2221 caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg
     2281 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2341 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcccgtccg cgcttaaact
     2401 ccaacatgga cgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2461 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     2521 gcaaaggtag cgttgccaat gatgttacag atgagatggt ccgtctcaac tggctgacgg
     2581 agtttatgcc tctcccgacc atcaagcatt ttatccgtac tcctgatgat gcgtggttac
     2641 tcaccaccgc gattcctggg aaaacagcct tccaggtatt agaagaatat cctgattcag
     2701 gtgaaaatat tgttgatgcg ctggccgtgt tcctgcgccg gttacattcg attcctgttt
     2761 gtaattgtcc ttttaacagc gatcgtgtat ttcgtcttgc tcaggcgcaa tcacgcatga
     2821 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2881 aagtctggaa agaaatgcac aagctcttgc cattctcacc ggattcagtc gtcactcatg
     2941 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     3001 ttggacgggt cggaatcgca gaccgttacc aggaccttgc cattctttgg aactgcctcg
     3061 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     3121 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaataa
//