LOCUS       BBa_K634011             2412 bp    DNA     linear       01-Jun-2024
DEFINITION  sacB + KanR
ACCESSION   BBa_K634011
VERSION     BBa_K634011.1  
FEATURES             Location/Qualifiers
     RBS             1..3
                     /label=RBS
     CDS             13..1434
                     /label=Coding sequence
     misc_feature    211..216
                     /label=Removed EcoRI site
     misc_feature    1..1437
                     /label=BBa_K322921
     CDS             1594..2412
                     /label=kanamycin resistance
     misc_feature    1446..2412
                     /label=BBa_P1003
ORIGIN
        1 gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact
       61 accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca
      121 tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa
      181 cagcaaaaaa atgaaaaata tcaagttcct gagttcgatt cgtccacaat taaaaatatc
      241 tcttctgcaa aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact
      301 gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg
      361 gatgacacat cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg
      421 aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta
      481 aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt
      541 ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa
      601 gttaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca
      661 atctttgacg gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac
      721 tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac
      781 aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct
      841 ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa
      901 cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt
      961 gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca
     1021 gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc
     1081 actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg
     1141 cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt
     1201 gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta
     1261 cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac
     1321 gcagacaaac aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca
     1381 tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataataatac
     1441 tagagctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct
     1501 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1561 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     1621 tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt tatatgggta taaatgggct
     1681 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1741 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1801 gtccgtctca actggctgac ggagtttatg cctctcccga ccatcaagca ttttatccgt
     1861 actcctgatg atgcgtggtt actcaccacc gcgattcctg ggaaaacagc cttccaggta
     1921 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggccgt gttcctgcgc
     1981 cggttacatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgtgt atttcgtctt
     2041 gctcaggcgc aatcacgcat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2101 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc acaagctctt gccattctca
     2161 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2221 aaattaatag gttgtattga tgttggacgg gtcggaatcg cagaccgtta ccaggacctt
     2281 gccattcttt ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2341 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2401 tttttctaat aa
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