BBa_K635002 1 BBa_K635002 Artificial Yeast Chromosome (Ura3 & Amp) 2011-09-26T11:00:00Z 2015-05-08T01:12:57Z The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in E.coli. The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively. Yeast transformants can be selected by using a uracil drop out medium and E.coli transformants may be selected using ampicillin resistance. false false _810_ 0 7191 9 Not in stock true The plasmid backbone contained a Gal1/10 bidirectional promoter between the EcoR1 and BamHI sites on the pRS316 plasmid. The plasmid had to be modified for biobrick compatibility by using the HindIII and SacI sites to digest and ligate a gene fragment with the corresponding HindIII and SacI sites, but which also contained the biobrick prefix and suffix in the correct orientation. false Arjun Athreya annotation2144979 1 URA3 range2144979 1 1611 1824 annotation2144980 1 CEN/ARS range2144980 1 2084 2602 annotation2144981 1 Ampicillin resistance range2144981 1 2734 3594 annotation2144982 1 psBR322 origin range2144982 1 3749 4368 annotation2144960 1 suffix range2144960 1 1 21 annotation2144962 1 prefix range2144962 1 4849 4870 BBa_K635002_sequence 1 gacgtcgccggcgatgatcatctcgaggttaagcgggatatcactcagcataatgttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtaggggggaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcgctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtcccattattgactatattaatttaacttcgagattaaacactcaaatcatatgtacgtaaatgaatattatgtcaaaaaatcaaaacgaccggcgtagaagagtttatacgaagggtcggacgaaaagacattgcaagtgggagatggaatcgtagggaagggaaacgtttatcaggagaaggttgttattattacagtctaggacatctctggtgtagtaggtgccaagatatgacaactgggttacgcagagggaacagtagatttgggtgtggcccacagtattagttggttagcattggaagtagagaaggtgggtacagagaaactcgttatttcggctattgttttagaaacagcgagaagcgttacagttgtcatgggaatcatataagaggtcatctatccctcgggaacgtactgttaagacgattgtagttttccggagatccaaggaaacaatgaagaagacggcggacgaagtttggcgattgttatggacccgggtggtgtggcacacgtaagcattacagacgggtaagacgataagacatatgtgggcgtctcatgacgttaaactgacataatggttacagtcgtttaaaagacagaagcttctcattttttaacatgaaccgcctattacggaaatcgccgaattgacacgggaggtacctttttagtcagttctataggtgtacacaaaaatcatttgtttaaaaccctggattacgaagttgattgaggtcattaaggaaccaccatgcttgtaggttacttcgtgtgttcaaacaaacgaaaagcacgtactataatttatcgaaccgtcgttgtcctgatcctactcatcgtcgtgcaaggaatatacatcgaaagctgtactaaatagaagcaaaggacgtccaaaaacaagacacgtcaacccaattcttatgacccgttaaagtacaaagaagttgtgatgtatacgcatatatatggttagattcagacacgaggaaggaagcaagaaggaagacaagcctctaatggcttagtttttttaaagtttctttggctttagttttttttcttattttttttttactacttaacttaacttttcgcaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatcctgcctagcgaacggacattgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaataaataaaaatacaaaacataaacctaaaatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgctattcattttacattttagtgtcctaaaagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctcgttctattttccatcataaacaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaaagaaaaaaatgaaagataaaaattaaatatataaatataattttttaaatttaatattaataaaaatatcgtgcactacttttcctgggtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaaagtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccgtcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgtaactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccccttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccggctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatggagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgaggatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgagccttaattgggagtgatttcccttgttttcgacccatggcccgggggggagctccagctgccatagctattcgaagagatcttcgccggcgcttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z