BBa_K635002
1
BBa_K635002
Artificial Yeast Chromosome (Ura3 & Amp)
2011-09-26T11:00:00Z
2015-05-08T01:12:57Z
The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest.
This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in E.coli. The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively.
Yeast transformants can be selected by using a uracil drop out medium and E.coli transformants may be selected using ampicillin resistance.
false
false
_810_
0
7191
9
Not in stock
true
The plasmid backbone contained a Gal1/10 bidirectional promoter between the EcoR1 and BamHI sites on the pRS316 plasmid. The plasmid had to be modified for biobrick compatibility by using the HindIII and SacI sites to digest and ligate a gene fragment with the corresponding HindIII and SacI sites, but which also contained the biobrick prefix and suffix in the correct orientation.
false
Arjun Athreya
annotation2144982
1
psBR322 origin
range2144982
1
3749
4368
annotation2144979
1
URA3
range2144979
1
1611
1824
annotation2144980
1
CEN/ARS
range2144980
1
2084
2602
annotation2144962
1
prefix
range2144962
1
4849
4870
annotation2144960
1
suffix
range2144960
1
1
21
annotation2144981
1
Ampicillin resistance
range2144981
1
2734
3594
BBa_K635002_sequence
1
gacgtcgccggcgatgatcatctcgaggttaagcgggatatcactcagcataatgttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtaggggggaagcggtcgaccgcattatcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcgctgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtcccattattgactatattaatttaacttcgagattaaacactcaaatcatatgtacgtaaatgaatattatgtcaaaaaatcaaaacgaccggcgtagaagagtttatacgaagggtcggacgaaaagacattgcaagtgggagatggaatcgtagggaagggaaacgtttatcaggagaaggttgttattattacagtctaggacatctctggtgtagtaggtgccaagatatgacaactgggttacgcagagggaacagtagatttgggtgtggcccacagtattagttggttagcattggaagtagagaaggtgggtacagagaaactcgttatttcggctattgttttagaaacagcgagaagcgttacagttgtcatgggaatcatataagaggtcatctatccctcgggaacgtactgttaagacgattgtagttttccggagatccaaggaaacaatgaagaagacggcggacgaagtttggcgattgttatggacccgggtggtgtggcacacgtaagcattacagacgggtaagacgataagacatatgtgggcgtctcatgacgttaaactgacataatggttacagtcgtttaaaagacagaagcttctcattttttaacatgaaccgcctattacggaaatcgccgaattgacacgggaggtacctttttagtcagttctataggtgtacacaaaaatcatttgtttaaaaccctggattacgaagttgattgaggtcattaaggaaccaccatgcttgtaggttacttcgtgtgttcaaacaaacgaaaagcacgtactataatttatcgaaccgtcgttgtcctgatcctactcatcgtcgtgcaaggaatatacatcgaaagctgtactaaatagaagcaaaggacgtccaaaaacaagacacgtcaacccaattcttatgacccgttaaagtacaaagaagttgtgatgtatacgcatatatatggttagattcagacacgaggaaggaagcaagaaggaagacaagcctctaatggcttagtttttttaaagtttctttggctttagttttttttcttattttttttttactacttaacttaacttttcgcaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatcctgcctagcgaacggacattgaatgtgcgcggagcatagaaaattactaccttattaaacccttaaatgagacacaaataaataaaaatacaaaacataaacctaaaatctttcatttatttcttccatcttctcaatgccttacttctttttttttatttgtttccaaattttttaaagttgtttttcgcatgaaatgtatatataaataatctgttcttttcgtctaatttatctatatgtaagctaattgctattcattttacattttagtgtcctaaaagcacacaccagaagatgtgtctgttctactttgttaagccgtaattatggactctcgtccttctcgttctattttccatcataaacaaccgctagggggatctcagaaaatgtagaagccttttgtttttgataaaaaagaaattaaagaaaaaaatgaaagataaaaattaaatatataaatataattttttaaatttaatattaataaaaatatcgtgcactacttttcctgggtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaaagtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccgtcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgtaactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccccttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccggctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatggagtgagtaatccgtggggtccgaaatgtgaaatacgaaggccgaggatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgagccttaattgggagtgatttcccttgttttcgacccatggcccgggggggagctccagctgccatagctattcgaagagatcttcgccggcgcttaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z