BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K638002 1 BBa_K638002 Reflectin A2 from Loligo pealei 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z n/a This brick contains Reflectin A2 from Loligo pealei false false _814_ 0 9698 9 Not in stock false n/a false Matthew Jones annotation2127518 1 reflectin A2 range2127518 1 19 708 annotation2127517 1 stop codon range2127517 1 709 711 annotation2127516 1 B0034 range2127516 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 BBa_K638102 1 BBa_K638102 Arabinose inducible Reflectin A2 generator 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z Arabinose inducible Reflectin A2 generator Released HQ 2013 Arabinose inducible Reflectin A2 generator - best used with Bw27783 - an Ecoli strain with constitutive expression of an Arabinose transporter. false false _814_ 0 9698 9 In stock false n/a false Matthew Jones component2222738 1 BBa_B0015 component2222727 1 BBa_B0034 component2222731 1 BBa_K638002 component2222725 1 BBa_I0500 annotation2222727 1 BBa_B0034 range2222727 1 1219 1230 annotation2222738 1 BBa_B0015 range2222738 1 1958 2086 annotation2222731 1 BBa_K638002 range2222731 1 1239 1949 annotation2222725 1 BBa_I0500 range2222725 1 1 1210 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K638002_sequence 1 aaagaggagaaatactagatgaatcgttatatgatgcgccatcgtccgatgtactcaaatatgtatcgtaccggtcgcaaatatcgtggcgtgatggaaccgatgtcacgcatgaccatggattttcagggccgctatatggattcgcagggccgcatggttgatccgcgctactatgattacggccgctgccatgattacgatcgttattacggtcgtagcatgtttaactatggcccgaacatggatggccagcgttacggtggctggatggatttcccggaacgctatatggacatgtcaggctaccagatggatatgcatggtcgttggatggatagccagggccgttattgcaatccgatgggccactcgtggtccaatcgtcagggttactatccgggctccaactacggtcgcaacatgtttaatccggaacgttacatggacatgagcggttaccagatggatatgcagggccgctggatggatatgggcggccgtcacgtgaacccgttttcgcatagcatgtacggtcgcaacatgtttaacccgtcctattttagtaatcgtcacatggataacccggaacgctacatggacatgagcggttatcagatggacatgcagggtcgctggatggatacccaaggccgctatatggacccgtcttggtcaaatatgtatgataactataatagttggtattaa BBa_B0034_sequence 1 aaagaggagaaa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K638102_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagagaaagaggagaaatactagatgaatcgttatatgatgcgccatcgtccgatgtactcaaatatgtatcgtaccggtcgcaaatatcgtggcgtgatggaaccgatgtcacgcatgaccatggattttcagggccgctatatggattcgcagggccgcatggttgatccgcgctactatgattacggccgctgccatgattacgatcgttattacggtcgtagcatgtttaactatggcccgaacatggatggccagcgttacggtggctggatggatttcccggaacgctatatggacatgtcaggctaccagatggatatgcatggtcgttggatggatagccagggccgttattgcaatccgatgggccactcgtggtccaatcgtcagggttactatccgggctccaactacggtcgcaacatgtttaatccggaacgttacatggacatgagcggttaccagatggatatgcagggccgctggatggatatgggcggccgtcacgtgaacccgttttcgcatagcatgtacggtcgcaacatgtttaacccgtcctattttagtaatcgtcacatggataacccggaacgctacatggacatgagcggttatcagatggacatgcagggtcgctggatggatacccaaggccgctatatggacccgtcttggtcaaatatgtatgataactataatagttggtattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z