BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K638103 1 BBa_K638103 Arabinose inducible Reflectin 1B generator 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z n/a Arabinose inducible Reflectin 1B generator - best used with Bw27783 - an Ecoli strain with constitutive expression of an arabinose transporter. false false _814_ 0 9698 9 It's complicated false n/a false Matthew Jones component2222721 1 BBa_B0015 component2222709 1 BBa_I0500 component2222711 1 BBa_B0034 component2222714 1 BBa_K638003 annotation2222721 1 BBa_B0015 range2222721 1 2123 2251 annotation2222711 1 BBa_B0034 range2222711 1 1219 1230 annotation2222709 1 BBa_I0500 range2222709 1 1 1210 annotation2222714 1 BBa_K638003 range2222714 1 1239 2114 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K638003 1 BBa_K638003 Reflectin 1B from Euprymna scolopes 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z n/a Reflectin 1B from Euprymna scolopes false false _814_ 0 9698 9 Not in stock false n/a false Matthew Jones annotation2137403 1 stop range2137403 1 876 876 annotation2137402 1 Reflectin 1B from Euprymna scolopes range2137402 1 21 876 annotation2137401 1 B0034 range2137401 1 1 12 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K638103_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagagaaagaggagaaatactagatgggatccatgaatcgcttcatgaataaatatcgcccgatgtttaacaacatgtattctaacatgtatcgtggtcgtaaccgcggcatgatggaaccgatgagccgcatgacgatggacttccagggccgttatatggattcacagggtcgcatggttgatccgcgttattatgattactatggccgttttaacgactacgatcgttattatggtcgtagtatgtttaactatggctggatgatggatggtgatcgctataatcgctataaccgttggatggattatccggaacgctatatggacatgagcggttaccaaatggacatgagcggtcgttggatggatatgcagggccgccactgcaatccgtattctcagtggggctataactataatcgccatggttattatccgaactacagctacggtcgtcacatgttttacccggaacgttggatggatatgagtggctatcagatggatatgcagggccgctatatggatcgttggggtcgctattgcaatccgttttcccagtacatgaactattatggccgttactggaattatccgggctacaatagctattataacagtcgcaatatgttctatccggaacgttattttgatatgtcaaactggcagatggatatgcaaggccgttggatggataaccagggtcgctattgcagcccgtattggaataattggtatggtcgtcagatgtattacccgtatcagaataattacttctacggccgttacgattatccgggcatggattatagcaattatcagatggacatgcagggtcgctacatggatcaatatggcatgaacgattattgttattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K638003_sequence 1 aaagaggagaaatactagatgggatccatgaatcgcttcatgaataaatatcgcccgatgtttaacaacatgtattctaacatgtatcgtggtcgtaaccgcggcatgatggaaccgatgagccgcatgacgatggacttccagggccgttatatggattcacagggtcgcatggttgatccgcgttattatgattactatggccgttttaacgactacgatcgttattatggtcgtagtatgtttaactatggctggatgatggatggtgatcgctataatcgctataaccgttggatggattatccggaacgctatatggacatgagcggttaccaaatggacatgagcggtcgttggatggatatgcagggccgccactgcaatccgtattctcagtggggctataactataatcgccatggttattatccgaactacagctacggtcgtcacatgttttacccggaacgttggatggatatgagtggctatcagatggatatgcagggccgctatatggatcgttggggtcgctattgcaatccgttttcccagtacatgaactattatggccgttactggaattatccgggctacaatagctattataacagtcgcaatatgttctatccggaacgttattttgatatgtcaaactggcagatggatatgcaaggccgttggatggataaccagggtcgctattgcagcccgtattggaataattggtatggtcgtcagatgtattacccgtatcagaataattacttctacggccgttacgattatccgggcatggattatagcaattatcagatggacatgcagggtcgctacatggatcaatatggcatgaacgattattgttattaa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z