LOCUS BBa_K638101 2455 bp DNA linear 22-Nov-2024 DEFINITION Arabinose inducible Reflectin A1 generator ACCESSION BBa_K638101 VERSION BBa_K638101.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 RBS 1239..1250 /label=B0034 CDS 1257..2315 /label=reflectin A1 misc_feature 2316..2318 /label=stop codon CDS 1239..2318 /label=BBa_K638001 stem_loop 2338..2381 /label=stem_loop misc_feature 2327..2406 /label=BBa_B0010 terminator 2327..2406 /label=BBa_B0010 stem_loop 2422..2441 /label=T7 TE misc_feature 2448..2448 /label=stop polyA_site 2442..2455 /label=polya misc_feature 2415..2455 /label=BBa_B0012 terminator 2415..2455 /label=BBa_B0012 terminator 2327..2455 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagagaa agaggagaaa tactagatgg 1261 gatccatgaa ccgttacctg aatcgtcaac gcctgtataa catgtaccgc aataaatatc 1321 gtggcgtaat ggaaccgatg tcgcgcatga cgatggattt ccagggtcgt tatatggatt 1381 cgcagggccg catggtagac ccgcgctact acgactacta cgggcgcatg catgatcatg 1441 atcgctatta tggccgtagt atgttcaatc agggtcattc aatggatagc cagcgttatg 1501 ggggctggat ggacaatccg gagcgctata tggacatgag cggttaccag atggatatgc 1561 aggggcgttg gatggatgcg cagggtcgct tcaacaatcc atttggtcaa atgtggcatg 1621 gtcgtcaggg tcattacccg gggtacatga gctcacattc catgtacggt cgcaatatgt 1681 ataacccata tcatagccat tatgcgagcc gccattttga ttcgccagag cgttggatgg 1741 atatgtctgg ttaccagatg gacatgcaag gtcgttggat ggataattac ggtcgctatg 1801 taaatccgtt caatcatcac atgtacggtc gcaacatgtg ctatccgtat gggaatcact 1861 attataaccg tcacatggaa catccggagc gttatatgga catgtccggc taccaaatgg 1921 acatgcaagg tcgctggatg gatacccatg gccgccattg caacccgttt ggccagatgt 1981 ggcataatcg ccatggctac tatccgggtc atccacatgg tcgcaacatg tttcagccgg 2041 aacgctggat ggatatgtct ggttatcaga tggatatgca ggggcgctgg atggataatt 2101 atggccgtta cgtgaatcca ttttcacata actatgggcg ccacatgaac tacccgggtg 2161 gccattataa ttaccatcac ggtcgttaca tgaaccaccc agaacgtcac atggatatga 2221 gctcctacca gatggacatg catggccgtt ggatggataa tcagggccgc tatattgata 2281 acttcgatcg taattattac gattaccaca tgtactaata ctagagccag gcatcaaata 2341 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2401 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata // LOCUS BBa_K638201 2455 bp DNA linear 22-Nov-2024 DEFINITION Arabinose inducible Poly-His Reflectin A1 generator ACCESSION BBa_K638201 VERSION BBa_K638201.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 RBS 1239..1250 /label=B0034 CDS 1257..2315 /label=reflectin A1 misc_feature 2316..2318 /label=stop codon CDS 1239..2318 /label=BBa_K638001 stem_loop 2338..2381 /label=stem_loop misc_feature 2327..2406 /label=BBa_B0010 terminator 2327..2406 /label=BBa_B0010 stem_loop 2422..2441 /label=T7 TE misc_feature 2448..2448 /label=stop polyA_site 2442..2455 /label=polya misc_feature 2415..2455 /label=BBa_B0012 terminator 2415..2455 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagagaa agaggagaaa tactagatgg 1261 gatccatgaa ccgttacctg aatcgtcaac gcctgtataa catgtaccgc aataaatatc 1321 gtggcgtaat ggaaccgatg tcgcgcatga cgatggattt ccagggtcgt tatatggatt 1381 cgcagggccg catggtagac ccgcgctact acgactacta cgggcgcatg catgatcatg 1441 atcgctatta tggccgtagt atgttcaatc agggtcattc aatggatagc cagcgttatg 1501 ggggctggat ggacaatccg gagcgctata tggacatgag cggttaccag atggatatgc 1561 aggggcgttg gatggatgcg cagggtcgct tcaacaatcc atttggtcaa atgtggcatg 1621 gtcgtcaggg tcattacccg gggtacatga gctcacattc catgtacggt cgcaatatgt 1681 ataacccata tcatagccat tatgcgagcc gccattttga ttcgccagag cgttggatgg 1741 atatgtctgg ttaccagatg gacatgcaag gtcgttggat ggataattac ggtcgctatg 1801 taaatccgtt caatcatcac atgtacggtc gcaacatgtg ctatccgtat gggaatcact 1861 attataaccg tcacatggaa catccggagc gttatatgga catgtccggc taccaaatgg 1921 acatgcaagg tcgctggatg gatacccatg gccgccattg caacccgttt ggccagatgt 1981 ggcataatcg ccatggctac tatccgggtc atccacatgg tcgcaacatg tttcagccgg 2041 aacgctggat ggatatgtct ggttatcaga tggatatgca ggggcgctgg atggataatt 2101 atggccgtta cgtgaatcca ttttcacata actatgggcg ccacatgaac tacccgggtg 2161 gccattataa ttaccatcac ggtcgttaca tgaaccaccc agaacgtcac atggatatga 2221 gctcctacca gatggacatg catggccgtt ggatggataa tcagggccgc tatattgata 2281 acttcgatcg taattattac gattaccaca tgtactaata ctagagccag gcatcaaata 2341 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2401 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //