BBa_K638101 1 BBa_K638101 Arabinose inducible Reflectin A1 generator 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z gibson assembly Released HQ 2013 Arabinose inducible Reflectin A1 generator false true _814_ 0 9698 9 In stock false n/a false Matthew Jones component2222742 1 BBa_I0500 component2222748 1 BBa_K638001 component2222744 1 BBa_B0034 component2222755 1 BBa_B0015 annotation2222755 1 BBa_B0015 range2222755 1 2327 2455 annotation2222748 1 BBa_K638001 range2222748 1 1239 2318 annotation2222744 1 BBa_B0034 range2222744 1 1219 1230 annotation2222742 1 BBa_I0500 range2222742 1 1 1210 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K638201 1 BBa_K638201 Arabinose inducible Poly-His Reflectin A1 generator 2011-09-18T11:00:00Z 2015-05-08T01:12:57Z This part was built from a codon optimised variant of the Reflectin A1 gene provided by Wendy Crookes. We added a his-tag by incorporating the part Part:BBa_K128005 into Gibson Assembly primers. Released HQ 2013 Arabinose inducible Poly-His Reflectin A1 generator produces a poly-histidine (his-tagged) form of reflectin A1. Bacteria expressing this construct will produce Reflectin A1 with a poly-histidine tag to enable purification with a his-affinity column used in conjunction with nickel or cobalt resins. The purified protein has optical properties, best viewed when made into a thin film. First the protein must be dissolved in an ionic solvent such as HFIP (hazardous - use in fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work, a razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a flow coater or spin coater device. true false _814_ 0 9698 9 Discontinued false The aim of this design was to produce a his-tagged variant of reflectin A1 so that the protein could be purified by his-affinity column purification. In addition, pBAD was used to allow us to induce expression of the protein with arabinose. The promoter and his-tag sequences were taken from the registry (see subpart list) as were the RBS and the terminator. We used Gibson Assembly to insert the reflectin gene, after we had checked for illeagal restriction sites. We found none, and thanks to the sequence being codon optimised for expression in E.coli had little trouble expressing and purifying the protein. false Matthew Jones component2134440 1 BBa_B0034 component2134447 1 BBa_B0012 component2134445 1 BBa_B0010 component2134444 1 BBa_K638001 component2134438 1 BBa_I0500 annotation2134445 1 BBa_B0010 range2134445 1 2327 2406 annotation2134447 1 BBa_B0012 range2134447 1 2415 2455 annotation2134440 1 BBa_B0034 range2134440 1 1219 1230 annotation2134444 1 BBa_K638001 range2134444 1 1239 2318 annotation2134438 1 BBa_I0500 range2134438 1 1 1210 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K638001 1 BBa_K638001 Reflectin A1 from Loligo 2011-08-03T11:00:00Z 2015-05-08T01:12:57Z n/a n/a false false _814_ 0 9698 9 Not in stock false n/a false Matthew Jones annotation2127498 1 stop codon range2127498 1 1078 1080 annotation2127496 1 B0034 range2127496 1 1 12 annotation2127497 1 reflectin A1 range2127497 1 19 1077 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K638001_sequence 1 aaagaggagaaatactagatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtactaa BBa_B0034_sequence 1 aaagaggagaaa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K638201_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagagaaagaggagaaatactagatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K638101_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagagaaagaggagaaatactagatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z