BBa_K638301 1 BBa_K638301 Arabinose inducible Reflectin A1-sfGFP fusion generator 2011-09-18T11:00:00Z 2015-05-08T01:12:57Z This composite part was built using gibson assembly to add Reflectin A1 between pBAD and sfGFP on an existing pBAD-sfGFP construct. BBa_I0500 - Registry Part BBa_B0034 - Registry Part BBa_K638001 - Codon optimised Reflectin A1 Supplied by Wendy Crookes BBa_I746916 - Registry Part BBa_B0010 - Registry Part BBa_B0012 - Registry Part E.coli transformed with this construct will produce a superfolder Green fluorescent Protein (sfGFP) fused variant of reflectin A1 under the control of pBAD, an arabinose inducible protein. There is currently (at the time of writing) no good assay for reflectin, in vivo. This construct allows reflectin to be localised within the cell. This part has been used to view reflectin containing inclusion bodies in E.coli and the sfGFP retained its fluorescence despite the fusion. false false _814_ 0 9698 9 It's complicated false We deliberately arranged the sfGFP part of the fusion on the C terminus of the protein as translation is known to proceed from N terminus to C terminus. This means that any sfGFP visualised in vivo must have been produced with reflectin A1 synthesised first, then the sfGFP component. false Matthew Jones annotation2135724 1 BBa_I746916 range2135724 1 2299 3015 annotation2135730 1 BBa_B0012 range2135730 1 3111 3151 annotation2135718 1 PC range2135718 1 1043 1114 annotation2135716 1 BBa_I0500 range2135716 1 1 1210 annotation2135717 1 AraC range2135717 1 1 1079 annotation2135731 1 T7 TE range2135731 1 3118 3137 annotation2135728 1 BBa_B0010 range2135728 1 3030 3109 annotation2135729 1 stem_loop range2135729 1 3041 3084 annotation2135723 1 BBa_K638001 range2135723 1 1237 2299 annotation2135722 1 reflectin A1 range2135722 1 1237 2299 annotation2135720 1 BBa_B0034 range2135720 1 1219 1230 annotation2135733 1 stop range2135733 1 3144 3144 annotation2135726 1 start range2135726 1 2299 2301 annotation2135725 1 superfolder GFP coding region range2135725 1 2299 3015 annotation2135719 1 Pbad range2135719 1 1080 1210 annotation2135732 1 polya range2135732 1 3138 3151 annotation2135727 1 stop range2135727 1 3013 3015 annotation2135721 1 conserved range2135721 1 1223 1226 BBa_K638301_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z