BBa_K638401 1 BBa_K638401 Arabinose inducible TorA Export Tagged Reflectin A1 Poly His generator 2011-09-18T11:00:00Z 2015-05-08T01:12:57Z Parts from the registry, Reflectin A1 DNA from Wendy Crookes, TorA sequence from paper: Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli Joanna D. Thomas1, Richard A. Daniel2, Jeff Errington2, Colin Robinson1,* The TorA sequence was incorporated into the construct using Gibson Assembly. Released HQ 2013 This construct is a Reflectin A1 protein with a TorA signal peptide on the N terminus, which has been shown to allow export of GFP via the twin arginine pathway. In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose. false false _814_ 0 9698 9 In stock false We used a TorA sequence from the literature that had been shown to cause GFP to be exported to the periplasm of e.coli. This sequence has also been checked for illegal restriction sites - using the tool A plasmid Editor (ApE) a restriction digest prediction revealed no sites for EcoRI, SpeI, XbaI or PstI. false Matthew Jones annotation2136121 1 polya range2136121 1 2569 2582 annotation2136107 1 BBa_I0500 range2136107 1 1 1210 annotation2136110 1 Pbad range2136110 1 1080 1210 annotation2136115 1 reflectin A1 range2136115 1 1366 2427 annotation2136120 1 T7 TE range2136120 1 2549 2568 annotation2136117 1 BBa_B0010 range2136117 1 2454 2533 annotation2136109 1 PC range2136109 1 1043 1114 annotation2136113 1 BBa_K638402 range2136113 1 1237 1365 annotation2136108 1 AraC range2136108 1 1 1079 annotation2136111 1 BBa_B0034 range2136111 1 1219 1230 annotation2136112 1 conserved range2136112 1 1223 1226 annotation2136116 1 BBa_K128005 range2136116 1 2428 2446 annotation2136118 1 stem_loop range2136118 1 2464 2508 annotation2136123 1 stop range2136123 1 2444 2446 annotation2136122 1 stop range2136122 1 2580 2580 annotation2136114 1 BBa_K638001 range2136114 1 1366 2427 annotation2136119 1 BBa_B0012 range2136119 1 2542 2582 BBa_K638401_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggcgaacaacgacttatttcaggcttctcggcgtcgctttctggcgcagctgggcggattaacggtggcgggtatgttgggcccgtcgctgttgactcctcgcagagcgacggcagcacaagccgcaatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtaccatcatcaccatcaccactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z