##gff-version 3
##sequence-region BBa_K639000 1 2248
BBa_K639000	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K639000	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K639000	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K639000	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K639000	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K639000	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K639000	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K639000	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K639000	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K639000	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K639000	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K639000	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K639000	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K639000	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K639000	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K639000	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K639000	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K639000	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K639000	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K639000	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K639000	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K639000	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K639000	.	engineered_region	1380	2248	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_K639000	.	terminator	2208	2248	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K639000	.	stem_loop	2215	2234	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K639000	.	stop_codon	2241	2241	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K639000	.	polyA_site	2235	2248	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K639000	.	engineered_region	2208	2248	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K639000	.	terminator	2120	2199	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K639000	.	stem_loop	2131	2174	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K639000	.	engineered_region	2120	2199	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K639000	.	engineered_region	1398	2111	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K639000	.	CDS	1398	2108	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K639000	.	sequence_alteration	1749	1749	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K639000	.	ribosome_entry_site	1380	1391	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K639000	.	conserved_region	1384	1387	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K639000
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggata
acatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcga
gggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttc
gcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactact
tgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgaccca
ggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgta
atgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatca
agcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgca
gctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtac
gaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata