LOCUS       BBa_K639003             2749 bp    DNA     linear       25-Nov-2024
DEFINITION  rrnB P1-LacI-pLac-mCherry plausible stress sensor
ACCESSION   BBa_K639003
VERSION     BBa_K639003.1  
FEATURES             Location/Qualifiers
     promoter        1..493
                     /label=BBa_K639002
     misc_feature    506..509
                     /label=conserved
     RBS             502..513
                     /label=BBa_B0034
     misc_feature    1609..1647
                     /label=LVA
     CDS             520..1647
                     /label=lacI-LVA
     misc_feature    520..1647
                     /label=BBa_C0012
     misc_feature    1648..1672
                     /label=Help:Barcodes
     CDS             520..1647
                     /label=BBa_C0012
     stem_loop       1692..1735
                     /label=stem_loop
     misc_feature    1681..1760
                     /label=BBa_B0010
     terminator      1681..1760
                     /label=BBa_B0010
     stem_loop       1776..1795
                     /label=T7 TE
     misc_feature    1802..1802
                     /label=stop
     polyA_site      1796..1809
                     /label=polya
     misc_feature    1769..1809
                     /label=BBa_B0012
     terminator      1769..1809
                     /label=BBa_B0012
     terminator      1681..1809
                     /label=BBa_B0015
     misc_feature    1820..1836
                     /label=lac O1
     promoter        1837..1842
                     /label=-35
     misc_feature    1843..1859
                     /label=lac O1
     promoter        1860..1865
                     /label=-10
     misc_feature    1818..1871
                     /label=BBa_R0011
     promoter        1818..1871
                     /label=BBa_R0011
     misc_feature    1885..1888
                     /label=conserved
     RBS             1881..1892
                     /label=BBa_B0034
     CDS             1899..2609
                     /label=mCherry
     misc_feature    2250..2250
                     /label=C->T (removing PstI site)
     misc_feature    1899..2612
                     /label=BBa_J06504
     stem_loop       2632..2675
                     /label=stem_loop
     misc_feature    2621..2700
                     /label=BBa_B0010
     terminator      2621..2700
                     /label=BBa_B0010
     stem_loop       2716..2735
                     /label=T7 TE
     misc_feature    2742..2742
                     /label=stop
     polyA_site      2736..2749
                     /label=polya
     misc_feature    2709..2749
                     /label=BBa_B0012
     terminator      2709..2749
                     /label=BBa_B0012
     misc_feature    1881..2749
                     /label=BBa_J06702
     misc_feature    502..2749
                     /label=BBa_K639000
ORIGIN
        1 acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg
       61 cacccatttc cctctactac aagaagaact gttacaagtg ctgccagagg gaacccggct
      121 ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac atgaagcccc
      181 ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc caggagctga
      241 acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt
      301 aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
      361 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
      421 ggtcagcggg gttctcctga gaactccggc agagaaagca aaaataaatg cttgactctg
      481 tagcgggaag gcgtactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta
      541 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
      601 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
      661 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt
      721 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
      781 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
      841 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt
      901 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg
      961 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa
     1021 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
     1081 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat
     1141 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc
     1201 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt
     1261 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt
     1321 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg
     1381 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
     1441 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
     1501 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1561 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac
     1621 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
     1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1801 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag
     1861 atactgagca catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
     1921 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1981 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     2041 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     2101 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     2161 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2221 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
     2281 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     2341 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     2401 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2461 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     2521 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     2581 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     2641 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2701 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//