##gff-version 3
##sequence-region BBa_K639003 1 2749
BBa_K639003	.	promoter	1	493	.	+	0	ID=BBa_K639002;Name=BBa_K639002
BBa_K639003	.	engineered_region	502	2749	.	+	0	ID=BBa_K639000;Name=BBa_K639000
BBa_K639003	.	promoter	1818	1871	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K639000
BBa_K639003	.	promoter	1837	1842	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K639003	.	promoter	1860	1865	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K639003	.	engineered_region	1818	1871	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K639003	.	operator	1843	1859	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K639003	.	operator	1820	1836	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K639003	.	CDS	520	1647	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K639000
BBa_K639003	.	engineered_region	520	1647	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K639003	.	engineered_tag	1648	1672	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K639003	.	tag	1609	1647	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K639003	.	CDS	520	1647	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K639003	.	terminator	1681	1809	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K639000
BBa_K639003	.	terminator	1769	1809	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K639003	.	polyA_site	1796	1809	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K639003	.	engineered_region	1769	1809	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K639003	.	stem_loop	1776	1795	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K639003	.	stop_codon	1802	1802	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K639003	.	terminator	1681	1760	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K639003	.	stem_loop	1692	1735	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K639003	.	engineered_region	1681	1760	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K639003	.	ribosome_entry_site	502	513	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K639000
BBa_K639003	.	conserved_region	506	509	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K639003	.	engineered_region	1881	2749	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_K639000
BBa_K639003	.	engineered_region	1899	2612	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K639003	.	sequence_alteration	2250	2250	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K639003	.	CDS	1899	2609	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K639003	.	terminator	2621	2700	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K639003	.	stem_loop	2632	2675	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K639003	.	engineered_region	2621	2700	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K639003	.	ribosome_entry_site	1881	1892	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K639003	.	conserved_region	1885	1888	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K639003	.	terminator	2709	2749	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K639003	.	polyA_site	2736	2749	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K639003	.	engineered_region	2709	2749	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K639003	.	stem_loop	2716	2735	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K639003	.	stop_codon	2742	2742	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K639003
acgtatcctacgcccgtggttaagaatgaaagagccgccagataccgttgtattgggttgcacccatttccctctacta
caagaagaactgttacaagtgctgccagagggaacccggctggtggattctggcgcagcgattgctcgccgaacggcct
ggttgttagaacatgaagccccggatgcaaaatctgccgatgcgaatattgccttttgtatggcaatgacgccaggagc
tgaacaattattgcccgttttacagcgttacggcttcgaaacgctcgaaaaactggcagttttaggctgatttggttga
atgttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccactgaca
cggaacaacggcaaacacgccgccgggtcagcggggttctcctgagaactccggcagagaaagcaaaaataaatgcttg
actctgtagcgggaaggcgtactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatg
tcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcg
ggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcg
ttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccg
atcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatct
tctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgc
actaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggta
cgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctc
ggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgac
tggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgcca
acgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtggg
atacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaa
accagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtga
aaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata