LOCUS BBa_K639003 2749 bp DNA linear 07-Nov-2024 DEFINITION rrnB P1-LacI-pLac-mCherry plausible stress sensor ACCESSION BBa_K639003 VERSION BBa_K639003.1 FEATURES Location/Qualifiers promoter 1..493 /label=BBa_K639002 misc_feature 506..509 /label=conserved RBS 502..513 /label=BBa_B0034 misc_feature 1609..1647 /label=LVA CDS 520..1647 /label=lacI-LVA misc_feature 520..1647 /label=BBa_C0012 misc_feature 1648..1672 /label=Help:Barcodes CDS 520..1647 /label=BBa_C0012 stem_loop 1692..1735 /label=stem_loop misc_feature 1681..1760 /label=BBa_B0010 terminator 1681..1760 /label=BBa_B0010 stem_loop 1776..1795 /label=T7 TE misc_feature 1802..1802 /label=stop polyA_site 1796..1809 /label=polya misc_feature 1769..1809 /label=BBa_B0012 terminator 1769..1809 /label=BBa_B0012 terminator 1681..1809 /label=BBa_B0015 misc_feature 1820..1836 /label=lac O1 promoter 1837..1842 /label=-35 misc_feature 1843..1859 /label=lac O1 promoter 1860..1865 /label=-10 misc_feature 1818..1871 /label=BBa_R0011 promoter 1818..1871 /label=BBa_R0011 misc_feature 1885..1888 /label=conserved RBS 1881..1892 /label=BBa_B0034 CDS 1899..2609 /label=mCherry misc_feature 2250..2250 /label=C->T (removing PstI site) misc_feature 1899..2612 /label=BBa_J06504 stem_loop 2632..2675 /label=stem_loop misc_feature 2621..2700 /label=BBa_B0010 terminator 2621..2700 /label=BBa_B0010 stem_loop 2716..2735 /label=T7 TE misc_feature 2742..2742 /label=stop polyA_site 2736..2749 /label=polya misc_feature 2709..2749 /label=BBa_B0012 terminator 2709..2749 /label=BBa_B0012 misc_feature 1881..2749 /label=BBa_J06702 misc_feature 502..2749 /label=BBa_K639000 ORIGIN 1 acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg 61 cacccatttc cctctactac aagaagaact gttacaagtg ctgccagagg gaacccggct 121 ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac atgaagcccc 181 ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc caggagctga 241 acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt 301 aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg 361 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg 421 ggtcagcggg gttctcctga gaactccggc agagaaagca aaaataaatg cttgactctg 481 tagcgggaag gcgtactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta 541 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 601 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 661 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 721 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 781 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 841 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 901 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 961 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 1021 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 1081 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 1141 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 1201 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 1261 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 1321 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 1381 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 1441 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 1501 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 1561 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 1621 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1801 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag 1861 atactgagca catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 1921 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 1981 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 2041 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 2101 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 2161 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 2221 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 2281 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 2341 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 2401 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 2461 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 2521 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 2581 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 2641 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2701 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata // LOCUS BBa_K639004 2749 bp DNA linear 07-Nov-2024 DEFINITION rrnB P1-LacI-pLac-mCherry plausible stress sensor ACCESSION BBa_K639004 VERSION BBa_K639004.1 FEATURES Location/Qualifiers promoter 1..493 /label=BBa_K639002 misc_feature 506..509 /label=conserved RBS 502..513 /label=BBa_B0034 misc_feature 1609..1647 /label=LVA CDS 520..1647 /label=lacI-LVA misc_feature 520..1647 /label=BBa_C0012 misc_feature 1648..1672 /label=Help:Barcodes CDS 520..1647 /label=BBa_C0012 stem_loop 1692..1735 /label=stem_loop misc_feature 1681..1760 /label=BBa_B0010 terminator 1681..1760 /label=BBa_B0010 stem_loop 1776..1795 /label=T7 TE misc_feature 1802..1802 /label=stop polyA_site 1796..1809 /label=polya misc_feature 1769..1809 /label=BBa_B0012 terminator 1769..1809 /label=BBa_B0012 terminator 1681..1809 /label=BBa_B0015 misc_feature 1820..1836 /label=lac O1 promoter 1837..1842 /label=-35 misc_feature 1843..1859 /label=lac O1 promoter 1860..1865 /label=-10 misc_feature 1818..1871 /label=BBa_R0011 promoter 1818..1871 /label=BBa_R0011 misc_feature 1885..1888 /label=conserved RBS 1881..1892 /label=BBa_B0034 CDS 1899..2609 /label=mCherry misc_feature 2250..2250 /label=C->T (removing PstI site) misc_feature 1899..2612 /label=BBa_J06504 stem_loop 2632..2675 /label=stem_loop misc_feature 2621..2700 /label=BBa_B0010 terminator 2621..2700 /label=BBa_B0010 stem_loop 2716..2735 /label=T7 TE misc_feature 2742..2742 /label=stop polyA_site 2736..2749 /label=polya misc_feature 2709..2749 /label=BBa_B0012 terminator 2709..2749 /label=BBa_B0012 misc_feature 1881..2749 /label=BBa_J06702 misc_feature 502..2749 /label=BBa_K639000 ORIGIN 1 acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg 61 cacccatttc cctctactac aagaagaact gttacaagtg ctgccagagg gaacccggct 121 ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac atgaagcccc 181 ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc caggagctga 241 acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt 301 aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg 361 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg 421 ggtcagcggg gttctcctga gaactccggc agagaaagca aaaataaatg cttgactctg 481 tagcgggaag gcgtactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta 541 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 601 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 661 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 721 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 781 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 841 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 901 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 961 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 1021 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 1081 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 1141 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 1201 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 1261 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 1321 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 1381 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 1441 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 1501 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 1561 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 1621 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1801 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag 1861 atactgagca catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 1921 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 1981 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 2041 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 2101 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 2161 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 2221 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 2281 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 2341 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 2401 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 2461 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 2521 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 2581 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 2641 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2701 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //