LOCUS BBa_K640006 4066 bp DNA linear 22-Dec-2024 DEFINITION Napthenic Acid Reporter System ACCESSION BBa_K640006 VERSION BBa_K640006.1 FEATURES Location/Qualifiers promoter 1..178 /label=BBa_K640008 misc_feature 191..194 /label=conserved RBS 187..198 /label=BBa_B0034 misc_feature 3228..3228 /label=C->T CDS 205..3279 /label=lacZ misc_feature 205..207 /label=START misc_feature 3277..3279 /label=STOP CDS 205..3279 /label=BBa_I732005 stem_loop 3299..3342 /label=stem_loop misc_feature 3288..3367 /label=BBa_B0010 terminator 3288..3367 /label=BBa_B0010 stem_loop 3383..3402 /label=T7 TE misc_feature 3409..3409 /label=stop polyA_site 3403..3416 /label=polya misc_feature 3376..3416 /label=BBa_B0012 terminator 3376..3416 /label=BBa_B0012 misc_feature 187..3416 /label=BBa_I732019 misc_feature 3425..3798 /label=BBa_K640003 oriT 3807..4066 /label=BBa_K640002 oriT 3425..4066 /label=BBa_K640004 ORIGIN 1 aaacgctcct gtttatgtgt gtcctgcggt gaaacggctg cgcaatccag tataggcagt 61 gctaaaagtg aggctatggc gattttcagg tcactccggg ctttcttgac aaggaaaaag 121 actggtatga gttcagagac ccgcgccgtc tcggccgaat aaaacaggaa tcagcaccta 181 ctagagaaag aggagaaata ctagatgacc atgattacgg attcactggc cgtcgtttta 241 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 301 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 361 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa 421 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag 481 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg 541 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa 601 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat 661 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt 721 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg 781 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac 841 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt 901 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt 961 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc 1021 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta 1081 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg 1141 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt 1201 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt 1261 cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg 1321 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat 1381 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat 1441 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc 1501 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg 1561 agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg 1621 tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc 1681 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc 1741 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc 1801 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa 1861 tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg 1921 gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat 1981 tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc 2041 acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta 2101 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc 2161 ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat 2221 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc 2281 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc 2341 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc 2401 gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg 2461 ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc 2521 gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac 2581 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag 2641 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct 2701 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc 2761 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa 2821 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta 2881 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact 2941 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc 3001 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg 3061 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc 3121 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg 3181 gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaatttca gctgagcgcc 3241 ggtcgctacc attaccagtt ggtctggtgt caaaaataat actagagcca ggcatcaaat 3301 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3361 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3421 agagcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga taccctgatt 3481 actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg gattgcccag 3541 acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac gtagtggtgc 3601 gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag gacgtctaca 3661 ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc cattaaggga 3721 cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt tgttgtattg 3781 tgggacgcag atacagtgta ctagagttaa ggtatacttt ccgctgcata accctgcttc 3841 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt 3901 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata 3961 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg 4021 gcggtgctca acgggaatcc tgctctgcga ggctggccga taagct //