BBa_K641002
1
BBa_K641002
{Pctx}{rbs.ffGFP}
2011-08-30T11:00:00Z
2015-05-08T01:12:58Z
Pctx is a promoter from a virulence island in Vibrio cholerae. The ffGFP is a widely used purely synthetic protein.
This part is a general reporter for any ToxR system. When ToxR dimerizes it binds to Pctx and activates Transcription of genes downstream of Pctx. By putting ffGFP downstream of Pctx the strength of the ToxR construct dimerization is reported by a corresponding strength of GFP expression.
true
false
_817_
0
9470
9
Discontinued
false
The only design considerations were tuning an rbs to the ffGFP so that expression levels could clearly be distinguished.
false
Spencer Scott
annotation2125695
1
BBa_I746916
range2125695
1
189
908
annotation2125690
1
BBa_K641000
range2125690
1
1
144
annotation2125692
1
start
range2125692
1
189
191
annotation2125691
1
BBa_K641001
range2125691
1
153
182
annotation2125693
1
superfolder GFP coding region
range2125693
1
189
908
annotation2125694
1
stop
range2125694
1
903
908
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z