BBa_K641006 1 BBa_K641006 LambdaRep (no start codon) 2011-08-31T11:00:00Z 2015-05-08T01:12:58Z The Lambda Repressor is from the Lambda Phage. This is the coding sequence for a Lambda Repressor which is a homodimer. It can be used to connect to ToxR to force dimerization of ToxR and thus subsequent activaiton of the ctx promoter. false false _817_ 0 9470 9 Not in stock false Had to ensure that the Repressor did not have a disruptive function to the cell. false Spencer Scott BBa_K641005 1 BBa_K641005 ToxR with nhe1 and gly-ser linkers 2011-08-31T11:00:00Z 2015-05-08T01:12:58Z ToxR comes from a virulence island of Vibrio Cholerae. This is the coding sequence for the ToxR protein that has an nhe1 restriction site before a double gly-ser linker that is used to connect many different types of dimerizing proteins to the N-terminal end of ToxR. This sequence will be the same for most ToxR systems unless changing the linker length. ToxR dimerizes when the protein attached to the N-terminus that sits in the periplasm dimerizes with another ToxR-dimerizing construct. false false _817_ 0 9470 9 Not in stock false There had to be a way to exchange N-terminal parts which is why the Nhe1 site is included after the native sequence of ToxR. Another thing to consider is the length of the linker desired in the system. A GSGS linker was used in our system as the preferred length. false Spencer Scott BBa_K641007 1 BBa_K641007 P_rffGH.rbs.ToxR-Nhe1.{gsgsgs}{LambdaRep} 2011-08-31T11:00:00Z 2015-05-08T01:12:58Z This composite part comes from several sources all listed in its respective basic parts. This composite part constitutively expresses a ToxR-LambdaRep construct. This ToxR construct will dimerize at a high affinity, because of Lambda Rep's dimerizing properties, making the cytoplasmic ToxR dimer an active transcription factor for the ctx Promoter. Thus, If this composite part is combined with a Pctx-GFP reporter there will be a high expression of GFP. true false _817_ 0 9470 9 Discontinued false The biggest design considerations were how to limit the amount of ToxR expressed while still allowing enough ToxR to be produced to see a high expression of GFP. The P_rffGH promoter, the rbs, and the plasmid we put it on (ColE1 origin) all contributed to the optimal expression level of the ToxR construct. false Spencer Scott component2125767 1 BBa_K641006 component2125765 1 BBa_K641004 component2125766 1 BBa_K641005 annotation2125766 1 BBa_K641005 range2125766 1 32 685 annotation2125767 1 BBa_K641006 range2125767 1 694 1434 annotation2125765 1 BBa_K641004 range2125765 1 1 25 BBa_K641004 1 BBa_K641004 rbs for ToxR 2011-08-31T11:00:00Z 2015-05-08T01:12:58Z This rbs was selected from a library in a tuning process. This rbs sits in front of ToxR after the PffGH promoter in the ToxR dimerization system. false false _817_ 0 9470 9 Not in stock false The rbs needed to allow a specific translation rate of ToxR as to not overwhelm the cell with ToxR false Spencer Scott BBa_K641005_sequence 1 atgttcggattaggacacaactcaaaagaaatctcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgtatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccggagtccgtcaatgaatacgcagaatcaagcagtgtgccttcatctgctaccgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgggatctgctagcggaagcggatcg BBa_K641006_sequence 1 accaagaaaaagccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaa BBa_K641007_sequence 1 gccacgcacaaaactacggtagcaatactagatgttcggattaggacacaactcaaaagaaatctcgatgagtcatattggtactaaattcattcttgctgaaaaatttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagccgtatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctcgcgaaaacgaagctgctcatgacatctctcagccggagtccgtcaatgaatacgcagaatcaagcagtgtgccttcatctgctaccgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgtttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgggatctgctagcggaagcggatcgtactagagaccaagaaaaagccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaa BBa_K641004_sequence 1 gccacgcacaaaactacggtagcaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z