LOCUS       BBa_K642004             1718 bp    DNA     linear       29-Nov-2024
DEFINITION  TetR repressor tagged with yBFP and a VP16 activation domain
ACCESSION   BBa_K642004
VERSION     BBa_K642004.1  
FEATURES             Location/Qualifiers
     CDS             1..621
                     /label=TetR ORF
     misc_feature    622..627
                     /label=XbaI/SpeI mixed site
     CDS             628..1326
                     /label=BFP ORF
     misc_feature    1327..1332
                     /label=XbaI/SpeI mixed site
     CDS             1333..1718
                     /label=VP16 Activation domain
ORIGIN
        1 atgtcaagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc ttctagtatg tccgaattga tcaaggaaaa catgcacatg
      661 aaattgtata tggaaggtac tgtcgacaac caccacttca aatgcacctc cgaaggtgaa
      721 ggtaaacctt atgaaggtac acaaaccatg agaatcaaag tcgtcgaagg tggtccattg
      781 ccatttgctt tcgacatttt ggccacatct ttcttgtatg gttccaaaac tttcatcaat
      841 cacacccaag gtattccaga cttcttcaaa caatctttcc ctgaaggttt cacttgggaa
      901 agagtcacca cctatgaaga tggtggtgtc ttgactgcta ctcaagacac atccttacaa
      961 gacggttgct tgatctataa cgtcaagatt agaggtgtca acttcacatc aaacggtcct
     1021 gtcatgcaaa aaaagacatt gggttgggaa gctttcaccg aaactttgta tcctgccgac
     1081 ggtggtttag aaggtagaaa cgacatggcc ttaaaattgg tcggtggtag tcacttgatt
     1141 gccaacatca aaacaaccta tagatccaaa aaacctgcca aaaacttgaa aatgcctggt
     1201 gtctattatg tcgactatag attggaaaga attaaggaag ccaacaacga aacttatgtc
     1261 gaacaacacg aagttgctgt cgccagatat tgtgacttgc cttcaaaatt gggtcacaaa
     1321 ttgaactcta gtgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag
     1381 ggcctgctcg atctcccgga cgacgacgcc cccgaagagg cggggctggc ggctccgcgc
     1441 ctgtcctttc tccccgcggg acacacgcgc agactgtcga cggccccccc gaccgatgtc
     1501 agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg
     1561 ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc
     1621 ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg
     1681 tttaccgatg cccttggaat tgacgagtac ggtgggtt
//