BBa_K642004 1 BBa_K642004 TetR repressor tagged with yBFP and a VP16 activation domain 2011-09-21T11:00:00Z 2015-05-08T01:12:58Z The TetR repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319046) that was submitted last year by the uOttawa team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. gene. The VP16 activation domain was PCR amplified from a plasmid. Tetracycline inducible repressor (no stop codon included) tagged upstream with yeast codon optimized blue fluorescent protein (no stop codon included) and a VP16 activation domain. false false _818_ 0 2810 418 In stock true Both TetR and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with the VP16 activation domain. false Wilson Lam, Matthew Orton annotation2138673 1 BFP ORF range2138673 1 628 1326 annotation2138674 1 XbaI/SpeI mixed site range2138674 1 1327 1332 annotation2138672 1 XbaI/SpeI mixed site range2138672 1 622 627 annotation2138671 1 TetR ORF range2138671 1 1 621 annotation2138675 1 VP16 Activation domain range2138675 1 1333 1718 BBa_K642004_sequence 1 atgtcaagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttctagtatgtccgaattgatcaaggaaaacatgcacatgaaattgtatatggaaggtactgtcgacaaccaccacttcaaatgcacctccgaaggtgaaggtaaaccttatgaaggtacacaaaccatgagaatcaaagtcgtcgaaggtggtccattgccatttgctttcgacattttggccacatctttcttgtatggttccaaaactttcatcaatcacacccaaggtattccagacttcttcaaacaatctttccctgaaggtttcacttgggaaagagtcaccacctatgaagatggtggtgtcttgactgctactcaagacacatccttacaagacggttgcttgatctataacgtcaagattagaggtgtcaacttcacatcaaacggtcctgtcatgcaaaaaaagacattgggttgggaagctttcaccgaaactttgtatcctgccgacggtggtttagaaggtagaaacgacatggccttaaaattggtcggtggtagtcacttgattgccaacatcaaaacaacctatagatccaaaaaacctgccaaaaacttgaaaatgcctggtgtctattatgtcgactatagattggaaagaattaaggaagccaacaacgaaacttatgtcgaacaacacgaagttgctgtcgccagatattgtgacttgccttcaaaattgggtcacaaattgaactctagtgcgtacagccgcgcgcgtacgaaaaacaattacgggtctaccatcgagggcctgctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacacacgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z