LOCUS       BBa_K642005             2183 bp    DNA     linear       26-Dec-2024
DEFINITION  LacI repressor tagged with yBFP and a VP16 activation domain
ACCESSION   BBa_K642005
VERSION     BBa_K642005.1  
FEATURES             Location/Qualifiers
     CDS             1..1086
                     /label=LacI ORF
     misc_feature    1087..1092
                     /label=XbaI/SpeI mixed site
     CDS             1093..1791
                     /label=BFP ORF
     misc_feature    1792..1797
                     /label=XbaI/SpeI mixed site
     CDS             1798..2183
                     /label=VP16 Activation domain
ORIGIN
        1 atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 actagatcta gtatgtccga attgatcaag gaaaacatgc acatgaaatt gtatatggaa
     1141 ggtactgtcg acaaccacca cttcaaatgc acctccgaag gtgaaggtaa accttatgaa
     1201 ggtacacaaa ccatgagaat caaagtcgtc gaaggtggtc cattgccatt tgctttcgac
     1261 attttggcca catctttctt gtatggttcc aaaactttca tcaatcacac ccaaggtatt
     1321 ccagacttct tcaaacaatc tttccctgaa ggtttcactt gggaaagagt caccacctat
     1381 gaagatggtg gtgtcttgac tgctactcaa gacacatcct tacaagacgg ttgcttgatc
     1441 tataacgtca agattagagg tgtcaacttc acatcaaacg gtcctgtcat gcaaaaaaag
     1501 acattgggtt gggaagcttt caccgaaact ttgtatcctg ccgacggtgg tttagaaggt
     1561 agaaacgaca tggccttaaa attggtcggt ggtagtcact tgattgccaa catcaaaaca
     1621 acctatagat ccaaaaaacc tgccaaaaac ttgaaaatgc ctggtgtcta ttatgtcgac
     1681 tatagattgg aaagaattaa ggaagccaac aacgaaactt atgtcgaaca acacgaagtt
     1741 gctgtcgcca gatattgtga cttgccttca aaattgggtc acaaattgaa ctctagtgcg
     1801 tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct gctcgatctc
     1861 ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc
     1921 gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct gggggacgag
     1981 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     2041 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     2101 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     2161 ggaattgacg agtacggtgg gtt
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