BBa_K642005 1 BBa_K642005 LacI repressor tagged with yBFP and a VP16 activation domain 2011-09-22T11:00:00Z 2015-05-08T01:12:58Z The LacI repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319046) that was submitted last year by the uOttawa team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The rtTA activation domain was PCR amplified from a plasmid obtained by our host lab. LacI repressor tagged upstream with yeast codon optimized blue fluorescent protein and an rtTA activation domain. false false _818_ 0 3073 9 It's complicated false Both TetR and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the rtTA activation domain. false Wilson Lam, Matthew Orton annotation2138718 1 VP16 Activation domain range2138718 1 1798 2183 annotation2138717 1 XbaI/SpeI mixed site range2138717 1 1792 1797 annotation2138716 1 BFP ORF range2138716 1 1093 1791 annotation2138715 1 XbaI/SpeI mixed site range2138715 1 1087 1092 annotation2138714 1 LacI ORF range2138714 1 1 1086 BBa_K642005_sequence 1 atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagactagatctagtatgtccgaattgatcaaggaaaacatgcacatgaaattgtatatggaaggtactgtcgacaaccaccacttcaaatgcacctccgaaggtgaaggtaaaccttatgaaggtacacaaaccatgagaatcaaagtcgtcgaaggtggtccattgccatttgctttcgacattttggccacatctttcttgtatggttccaaaactttcatcaatcacacccaaggtattccagacttcttcaaacaatctttccctgaaggtttcacttgggaaagagtcaccacctatgaagatggtggtgtcttgactgctactcaagacacatccttacaagacggttgcttgatctataacgtcaagattagaggtgtcaacttcacatcaaacggtcctgtcatgcaaaaaaagacattgggttgggaagctttcaccgaaactttgtatcctgccgacggtggtttagaaggtagaaacgacatggccttaaaattggtcggtggtagtcacttgattgccaacatcaaaacaacctatagatccaaaaaacctgccaaaaacttgaaaatgcctggtgtctattatgtcgactatagattggaaagaattaaggaagccaacaacgaaacttatgtcgaacaacacgaagttgctgtcgccagatattgtgacttgccttcaaaattgggtcacaaattgaactctagtgcgtacagccgcgcgcgtacgaaaaacaattacgggtctaccatcgagggcctgctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacacacgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z