BBa_K642007 1 BBa_K642007 Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP 2011-10-23T11:00:00Z 2015-05-08T01:12:58Z The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039. This is the distal region of the mid length Adh1 promoter (BBa_K319005) submitted last year by the uOttawa team fused to the proximal region of the Gal10/1 promoter. It is a constitutively expressing chimeric promoter. It is also tagged with the reporter yeGFP (BBa_K319039) also submitted by the uOttawa team last year. false false _818_ 0 3073 9 It's complicated true The yeGFP part from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon to the reverse primer. false Ian Roney, Matthew Orton annotation2160467 1 XbaI/SpeI mixed site range2160467 1 517 522 annotation2160468 1 Gal1 proximal promoter range2160468 1 523 729 annotation2160469 1 XbaI/SpeI mixed site range2160469 1 730 735 annotation2160465 1 Adh1 distal promoter range2160465 1 1 516 annotation2160466 1 yeGFP range2160466 1 736 1446 BBa_K642007_sequence 1 tccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgaactagattagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaactataaactagaatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z