BBa_K643000
1
BBa_K643000
CDS7 cadmium sulfate binding peptide coding sequence
2011-09-24T11:00:00Z
2015-07-21T02:29:26Z
This part was synthesized de novo based on the information in the original paper. It was PCR'd out of the synthesized plasmid with RFC 23 overhangs and ligated into pSB1C3 vector.
This sequence codes for a short peptide that's been reported to bind to cadmium sulfate and nucleate quantum dot formation. (C. Mi et al. / Journal of Biotechnology 153 (2011) 125???132)
Peptide sequence: N-GDVHHHGRHGAEHADI-C
false
false
_819_
4206
6460
9
It's complicated
true
Original coding sequence in the referenced paper had NcoI and BamHI sites surrounding the coding sequence for cloning into pET28b IPTG inducible vector. For our purposes we eliminated the restriction sites and instead replaced them with standard biobricking prefix and suffix.
false
Sung won Lim
BBa_K643000_sequence
1
gccatcatcatcatcatcacggcgatgtgcatcatcatggccgccacggcgcggaacatgcggatatttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z