BBa_K238013 1 BLp Exact promoter of the blue light receptor 2009-08-01T11:00:00Z 2015-05-08T01:11:36Z E.coli strain MC4100 ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009) false false _332_ 0 4771 9 It's complicated true none false Annelien Verfaillie annotation2014614 1 transcription start range2014614 1 64 65 annotation2014612 1 inverted repeat part 1 range2014612 1 53 59 annotation2014610 1 -10 box range2014610 1 52 58 annotation2014609 1 -35 box range2014609 1 28 33 annotation2014613 1 inverted repeat part 2 range2014613 1 67 73 annotation2014611 1 spacer range2014611 1 34 51 BBa_K643001 1 BBa_K643001 Blue Light Promoter controlling Chloramphenicol Resistance 2011-09-26T11:00:00Z 2015-05-08T01:12:58Z Blue Light Promoter BBa_K238013 From K.U. Leuven 2009 Chloramphenicol Resistance BBa_P1004 From Knight Lab, MIT 2006 Blue Light Promoter (BLP) controlling Chloramphenicol Resistance (Chlor) on pSB1A3 vector BBa_P1004 (Chlor) BBa_K238013 (BLP) Reporter ycgF responds to blue light changing its conformation and dimerizing with ycgE molecules. Because of the dimerization, ycgF releases from the promoter region. Consequently, ycgF no longer represses gene transcription. Downstream, the gene for chloramphenicol resistance is now accessible to transcription enzymes. Thus, blue light initializes chloramphenicol resistance. false false _819_ 0 9448 9 It's complicated true The use of the psB1A3 as opposed to the psB1C3 backbone: The device produces chloramphenicol resistance in the cells. The backbone pSB1C3 has chloramphenicol resistance on its own and, if used as our backbone, would be redundant. It would not be possible to screen which cells were expressing the blue light promoter because chloramphenicol resistance would not be a guaranteed indicator of BLP expression. false Alison Acevedo, Peter (Renxuan) Liu component2144542 1 BBa_P1004 component2144540 1 BBa_K238013 annotation2144542 1 BBa_P1004 range2144542 1 95 863 annotation2144540 1 BBa_K238013 range2144540 1 1 86 BBa_P1004 1 cmR chloramphenicol resistance cassette 2006-02-03T12:00:00Z 2015-05-08T01:14:11Z Released HQ 2013 same part as BBa_P1002 except in reverse orientation. false false _41_6_ 0 126 45 In stock false true Reshma Shetty annotation1897114 1 cmR range1897114 1 107 769 BBa_K238013_sequence 1 attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttt BBa_P1004_sequence 1 gttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa BBa_K643001_sequence 1 attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttttactagaggttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z