BBa_K644000 1 BBa_K644000 Extracellular Domain of E-Cadherin (Mouse) 2011-08-30T11:00:00Z 2015-05-08T01:12:58Z Mus musculus (mouse) Cadherin is an adhesive transmembrane and calcium dependent protein that is expressed in many mammalian tissue cells and plays a role in keeping tissues together. It has a domain that anchors the protein to the cytoskeleton of the mammalian cells, an intercellular domain, and five extracellular domain repeats. With the presence of calcium ions, the extracellalur domains become rigid and are able to form homodimers with the same type of cadherin or form heterodimers with different types of cadherin, hence creating cell to cell adhesion. false false _820_ 0 10307 9 It's complicated false Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3). false Kingsley Lim BBa_K644000_sequence 1 ctcagaagacagaaacgagactgggtcatccctcccatcagctgccccgaaaatgaaaagggtgagttcccaaagaacctggttcagatcaaatccaacagggacaaagaaacaaaggttttctacagcatcaccggccaaggagctgacaaaccccccgttggcgttttcatcattgagagggagacaggctggctgaaagtgacacagcctctggatagagaagccattgccaagtacatcctctattctcatgccgtgtcatcaaatggggaagcggtggaggatcccatggagatagtgatcacagtgacagatcagaatgacaacaggccagagtttacccaggaggtgtttgagggatccgttgcagaaggcgctgttccaggaacctccgtgatgaaggtctcagccaccgatgcagacgatgacgtcaacacctacaacgctgccatcgcctacaccatcgtcagccaggatcctgagctgcctcacaaaaacatgttcactgtcaatagggacaccggggtcatcagtgtgctcacctctgggctggaccgagagagttaccctacatacactctggtggttcaggctgctgaccttcaaggcgaaggcttgagcacaacagccaaggctgtgatcactgtcaaggatattaatgacaacgctcctgtcttcaacccgagcacgtatcagggtcaagtgcctgagaatgaggtcaatgcccggatcgccacactcaaagtgaccgatgatgatgcccccaacactccggcgtggaaagctgtgtacaccgtagtcaacgatcctgaccagcagttcgttgtcgtcacagaccccacgaccaatgatggcattttgaaaacagccaagggcttggattttgaggccaagcagcaatacatccttcatgtgagagtggagaacgaggaaccctttgaggggtctcttgtcccttccacagccactgtcactgtggacgtggtagacgtgaatgaagcccccatctttatgcctgcggagaggagagtcgaagtgcccgaagactttggtgtgggtcaggaaatcacatcttataccgctcgagagccggacacgttcatggatcagaagatcacgtatcggatttggagggacactgccaactggctggagattaacccagagactggtgccattttcacgcgcgctgagatggacagagaagacgctgagcatgtgaagaacagcacatatgtagctctcatcatcgccacagatgatggttcacccattgccactggcacgggcactcttctcctggtcctgttagacgtcaatgacaacgctcccatcccagaacctcgaaacatgcagttctgccagaggaacccacagcctcatatcatcaccatcttggatccagaccttccccccaacacgtccccctttactgctgagctaacccatggggccagcgtcaactggaccattgagtataatgacgcagctcaagaatctctcattttgcaaccaagaaaggacttagagattggcgaatacaaaatccatctcaagctcgcggataaccagaacaaagaccaggtgaccacgttggacgtccatgtgtgtgactgtgaagggacggtcaacaactgcatgaaggcgggaatcgtggcagcaggattgcaagttcctgccatcctcggaatccttggagggatcctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z