BBa_K646004 1 BBa_K646004 T7 promoter with Hemeoxygenase 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z BBa_K646000 and BBa_I712074 Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin. false false _822_ 0 10455 9 Not in stock true None. false David Dubow component2152632 1 BBa_I712074 component2152635 1 BBa_K646000 annotation2152635 1 BBa_K646000 range2152635 1 55 798 annotation2152632 1 BBa_I712074 range2152632 1 1 46 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K646000 1 HO1 Hemeoxygenase with RBS 2011-09-28T11:00:00Z 2015-05-08T01:12:58Z Http://www.ncbi.nlm.nih.gov/gene/953483 Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _822_ 0 10455 9 In stock true The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation. false Edward Moh annotation2147982 1 RBS range2147982 1 9 14 annotation2147983 1 Heme Oxygenase 1 range2147983 1 22 744 BBa_K646000_sequence 1 ctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_K646004_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z