BBa_K646005 1 BBa_K646005 T7 promoter with Heme oxygenase and Deinococcus radiodurans bacteriophytochrome 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z Bacterial phytochrome isolated from genomic DNA, T7 from 2011 kit plate. This is an assembly of an Deinococcus radiodurans isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. Sequence from National Center for Biotechnology. false false _822_ 0 10455 9 Not in stock false Continuation of attempts to create BBa_K497007. false David Dubow component2219978 1 BBa_I712074 component2219981 1 BBa_K646000 component2219984 1 BBa_K646002 annotation2219981 1 BBa_K646000 range2219981 1 55 798 annotation2219984 1 BBa_K646002 range2219984 1 807 3087 annotation2219978 1 BBa_I712074 range2219978 1 1 46 BBa_K646002 1 DR-Bph Deinococcus radiodurans bacteriophytochrome 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z Sequence from National Center for Biotechnology. The Deinococcus radiodurans bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. This part also has a ribosome binding site (AGGAGG) located 6 base pairs upstream from the ATG start codon. false false _822_ 0 10455 9 Not in stock false Contains an EcoR1 site that was changed by Site directed mutagenesis using a silent mutation. false David Dubow annotation2147995 1 DR-Bph range2147995 1 13 2280 annotation2147994 1 RBS range2147994 1 1 6 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K646000 1 HO1 Hemeoxygenase with RBS 2011-09-28T11:00:00Z 2015-05-08T01:12:58Z Http://www.ncbi.nlm.nih.gov/gene/953483 Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _822_ 0 10455 9 In stock true The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation. false Edward Moh annotation2147982 1 RBS range2147982 1 9 14 annotation2147983 1 Heme Oxygenase 1 range2147983 1 22 744 BBa_K646000_sequence 1 ctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K646002_sequence 1 aggagggctgctatgagccgggacccgttgcccttttttccaccgctttaccttggtggcccggaaattaccaccgagaactgcgagcgcgagccgattcatattcccggcagcatccagccgcacggcgccctgctcactgccgacgggcacagcggcgaggtgctccagatgagcctcaacgcggccacttttctgggacaggaacccacagtgctgcgcggacagaccctcgccgcactgctgcccgagcagtggcccgcgctgcaagcggccctgccccccggctgccccgacgccctgcaataccgcgcaacgctggactggcctgccgccgggcacctttcgctgacggtgcaccgggtcggcgagttgctgattctggagttcgagccgacggaggcctgggacagcaccgggccgcacgcgctgcgcaacgcgatgttcgcgctcgaaagtgcccccaacctgcgggcgctggccgaggtggcgacccagacggtccgcgagctgacgggctttgaccgggtgatgctctacaaatttgcccccgacgccaccggcgaagtgattgccgaggcccgccgtgaggggctgcacgcctttctgggccaccgttttcccgcgtcggacattccggcgcaggcccgcgcgctctacacccggcacctgctgcgcctgaccgccgacacccgcgccgccgccgtgccgctcgatcccgtcctcaacccgcagacgaatgcgcccaccccgctgggcggcgccgtgctgcgcgccacctcgcccatgcacatgcagtacctgcggaacatgggcgtcgggtcgagcctgtcggtgtcggtggtggtcggcggccagctctggggcctgatcgcctgccaccaccagacgccctacgtgttgccgcccgacctgcgaaccacgctcgaatacctgggccgcttgctgagcctgcaagttcaggtcaaggaagcggcggacgtggcggcctttcgccagagcctgcgggagcaccacgcgcgggtggccctcgcggcggcgcactcgctctcgccgcacgacaccctcagtgacccggcgcttgacctgctgggcctgatgcgggccgggggcctgattctgcgtttcgagggccgctggcagacgttgggtgaagtgccgcctgccccggcggtggacgcgctgctggcgtggctcgaaacccagccgggcgccctggtccagaccgacgcgctgggccaactgtggcccgccggcgccgatctcgcccccagcgcagcgggcctgctcgccatcagcgtgggcgagggctggtcggagtgcctcgtctggctgcggcccgaactgcggctggaggtcgcctggggcggggccactcctgaccaggcgaaagacgacctcgggccgcgccactcattcgacacctacctcgaagaaaaacgcggctacgccgagccctggcatcccggcgaaatcgaggaggcgcaggatctacgtgacacattgaccggggcgctgggcgagcgcctgagcgtgattcgtgacctcaaccgggcgctcacacagtcgaacgccgagtggcggcagtacggcttcgttatcagccaccacatgcaggagccggtgcggctcatctcgcagttcgccgagttgctgacgcgccagccccgcgcccaggacgggtctccggactctccgcagaccgagcgcatcaccggctttctgctgcgcgaaacgtcgcgcctgcgcagcctgacgcaagacctccacacctacaccgcgctgctctcggcaccgccgccggtgcgccgccccacgccgctgggccgcgtggtggacgatgtgctgcaagacctcgaaccccgcattgccgacaccggagcgagcatcgaggtggcgcccgagttgcccgtcatcgctgccgacgctggcctgctgcgcgacctgctgctgcatctgatcggcaacgcgctgacgtttggtggcccggagccgcgtattgccgtaaggaccgaacggcaaggcgcgggttggtctatcgcggtcagtgaccagggcgctggcatcgcgcccgagtatcaggaacgaatctttctgctgtttcagcggctcggttcgctcgatgaggcgctgggcaacggcctgggcctgccgctgtgccgcaagatcgccgaactgcatggcggcaccctgaccgtggagtccgcgccaggcgagggcagcaccttccgttgctggctgcccgatgctgggcctcttccgggagccgccgatgcctgat BBa_K646005_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagagaggagggctgctatgagccgggacccgttgcccttttttccaccgctttaccttggtggcccggaaattaccaccgagaactgcgagcgcgagccgattcatattcccggcagcatccagccgcacggcgccctgctcactgccgacgggcacagcggcgaggtgctccagatgagcctcaacgcggccacttttctgggacaggaacccacagtgctgcgcggacagaccctcgccgcactgctgcccgagcagtggcccgcgctgcaagcggccctgccccccggctgccccgacgccctgcaataccgcgcaacgctggactggcctgccgccgggcacctttcgctgacggtgcaccgggtcggcgagttgctgattctggagttcgagccgacggaggcctgggacagcaccgggccgcacgcgctgcgcaacgcgatgttcgcgctcgaaagtgcccccaacctgcgggcgctggccgaggtggcgacccagacggtccgcgagctgacgggctttgaccgggtgatgctctacaaatttgcccccgacgccaccggcgaagtgattgccgaggcccgccgtgaggggctgcacgcctttctgggccaccgttttcccgcgtcggacattccggcgcaggcccgcgcgctctacacccggcacctgctgcgcctgaccgccgacacccgcgccgccgccgtgccgctcgatcccgtcctcaacccgcagacgaatgcgcccaccccgctgggcggcgccgtgctgcgcgccacctcgcccatgcacatgcagtacctgcggaacatgggcgtcgggtcgagcctgtcggtgtcggtggtggtcggcggccagctctggggcctgatcgcctgccaccaccagacgccctacgtgttgccgcccgacctgcgaaccacgctcgaatacctgggccgcttgctgagcctgcaagttcaggtcaaggaagcggcggacgtggcggcctttcgccagagcctgcgggagcaccacgcgcgggtggccctcgcggcggcgcactcgctctcgccgcacgacaccctcagtgacccggcgcttgacctgctgggcctgatgcgggccgggggcctgattctgcgtttcgagggccgctggcagacgttgggtgaagtgccgcctgccccggcggtggacgcgctgctggcgtggctcgaaacccagccgggcgccctggtccagaccgacgcgctgggccaactgtggcccgccggcgccgatctcgcccccagcgcagcgggcctgctcgccatcagcgtgggcgagggctggtcggagtgcctcgtctggctgcggcccgaactgcggctggaggtcgcctggggcggggccactcctgaccaggcgaaagacgacctcgggccgcgccactcattcgacacctacctcgaagaaaaacgcggctacgccgagccctggcatcccggcgaaatcgaggaggcgcaggatctacgtgacacattgaccggggcgctgggcgagcgcctgagcgtgattcgtgacctcaaccgggcgctcacacagtcgaacgccgagtggcggcagtacggcttcgttatcagccaccacatgcaggagccggtgcggctcatctcgcagttcgccgagttgctgacgcgccagccccgcgcccaggacgggtctccggactctccgcagaccgagcgcatcaccggctttctgctgcgcgaaacgtcgcgcctgcgcagcctgacgcaagacctccacacctacaccgcgctgctctcggcaccgccgccggtgcgccgccccacgccgctgggccgcgtggtggacgatgtgctgcaagacctcgaaccccgcattgccgacaccggagcgagcatcgaggtggcgcccgagttgcccgtcatcgctgccgacgctggcctgctgcgcgacctgctgctgcatctgatcggcaacgcgctgacgtttggtggcccggagccgcgtattgccgtaaggaccgaacggcaaggcgcgggttggtctatcgcggtcagtgaccagggcgctggcatcgcgcccgagtatcaggaacgaatctttctgctgtttcagcggctcggttcgctcgatgaggcgctgggcaacggcctgggcctgccgctgtgccgcaagatcgccgaactgcatggcggcaccctgaccgtggagtccgcgccaggcgagggcagcaccttccgttgctggctgcccgatgctgggcctcttccgggagccgccgatgcctgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z