BBa_K646003 1 BBa_K646003 T7 promoter with Hemeoxygenase 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z BBa_K646000 and BBa_I719005 Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin. false false _822_ 0 10455 9 Not in stock true None. false David Dubow component2152628 1 BBa_I719005 component2152631 1 BBa_K646000 annotation2152628 1 BBa_I719005 range2152628 1 1 23 annotation2152631 1 BBa_K646000 range2152631 1 32 775 BBa_K646006 1 BBa_K646006 T7 promoter with Heme oxygenase and Deinococcus radiodurans bacteriophytochrome 2011-09-29T11:00:00Z 2015-05-08T01:12:59Z Sequence from National Center for Biotechnology. This is an assembly of an Deinococcus radiodurans isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. false false _822_ 0 10455 9 Not in stock false Duplicate of part BBa_K646006 using a different T7 promoter. false David Dubow component2219992 1 BBa_K646002 component2219989 1 BBa_K646003 annotation2219992 1 BBa_K646002 range2219992 1 784 3064 annotation2219989 1 BBa_K646003 range2219989 1 1 775 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K646000 1 HO1 Hemeoxygenase with RBS 2011-09-28T11:00:00Z 2015-05-08T01:12:58Z Http://www.ncbi.nlm.nih.gov/gene/953483 Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _822_ 0 10455 9 In stock true The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation. false Edward Moh annotation2147983 1 Heme Oxygenase 1 range2147983 1 22 744 annotation2147982 1 RBS range2147982 1 9 14 BBa_K646002 1 DR-Bph Deinococcus radiodurans bacteriophytochrome 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z Sequence from National Center for Biotechnology. The Deinococcus radiodurans bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. This part also has a ribosome binding site (AGGAGG) located 6 base pairs upstream from the ATG start codon. false false _822_ 0 10455 9 Not in stock false Contains an EcoR1 site that was changed by Site directed mutagenesis using a silent mutation. false David Dubow annotation2147995 1 DR-Bph range2147995 1 13 2280 annotation2147994 1 RBS range2147994 1 1 6 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K646006_sequence 1 taatacgactcactatagggagatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagagaggagggctgctatgagccgggacccgttgcccttttttccaccgctttaccttggtggcccggaaattaccaccgagaactgcgagcgcgagccgattcatattcccggcagcatccagccgcacggcgccctgctcactgccgacgggcacagcggcgaggtgctccagatgagcctcaacgcggccacttttctgggacaggaacccacagtgctgcgcggacagaccctcgccgcactgctgcccgagcagtggcccgcgctgcaagcggccctgccccccggctgccccgacgccctgcaataccgcgcaacgctggactggcctgccgccgggcacctttcgctgacggtgcaccgggtcggcgagttgctgattctggagttcgagccgacggaggcctgggacagcaccgggccgcacgcgctgcgcaacgcgatgttcgcgctcgaaagtgcccccaacctgcgggcgctggccgaggtggcgacccagacggtccgcgagctgacgggctttgaccgggtgatgctctacaaatttgcccccgacgccaccggcgaagtgattgccgaggcccgccgtgaggggctgcacgcctttctgggccaccgttttcccgcgtcggacattccggcgcaggcccgcgcgctctacacccggcacctgctgcgcctgaccgccgacacccgcgccgccgccgtgccgctcgatcccgtcctcaacccgcagacgaatgcgcccaccccgctgggcggcgccgtgctgcgcgccacctcgcccatgcacatgcagtacctgcggaacatgggcgtcgggtcgagcctgtcggtgtcggtggtggtcggcggccagctctggggcctgatcgcctgccaccaccagacgccctacgtgttgccgcccgacctgcgaaccacgctcgaatacctgggccgcttgctgagcctgcaagttcaggtcaaggaagcggcggacgtggcggcctttcgccagagcctgcgggagcaccacgcgcgggtggccctcgcggcggcgcactcgctctcgccgcacgacaccctcagtgacccggcgcttgacctgctgggcctgatgcgggccgggggcctgattctgcgtttcgagggccgctggcagacgttgggtgaagtgccgcctgccccggcggtggacgcgctgctggcgtggctcgaaacccagccgggcgccctggtccagaccgacgcgctgggccaactgtggcccgccggcgccgatctcgcccccagcgcagcgggcctgctcgccatcagcgtgggcgagggctggtcggagtgcctcgtctggctgcggcccgaactgcggctggaggtcgcctggggcggggccactcctgaccaggcgaaagacgacctcgggccgcgccactcattcgacacctacctcgaagaaaaacgcggctacgccgagccctggcatcccggcgaaatcgaggaggcgcaggatctacgtgacacattgaccggggcgctgggcgagcgcctgagcgtgattcgtgacctcaaccgggcgctcacacagtcgaacgccgagtggcggcagtacggcttcgttatcagccaccacatgcaggagccggtgcggctcatctcgcagttcgccgagttgctgacgcgccagccccgcgcccaggacgggtctccggactctccgcagaccgagcgcatcaccggctttctgctgcgcgaaacgtcgcgcctgcgcagcctgacgcaagacctccacacctacaccgcgctgctctcggcaccgccgccggtgcgccgccccacgccgctgggccgcgtggtggacgatgtgctgcaagacctcgaaccccgcattgccgacaccggagcgagcatcgaggtggcgcccgagttgcccgtcatcgctgccgacgctggcctgctgcgcgacctgctgctgcatctgatcggcaacgcgctgacgtttggtggcccggagccgcgtattgccgtaaggaccgaacggcaaggcgcgggttggtctatcgcggtcagtgaccagggcgctggcatcgcgcccgagtatcaggaacgaatctttctgctgtttcagcggctcggttcgctcgatgaggcgctgggcaacggcctgggcctgccgctgtgccgcaagatcgccgaactgcatggcggcaccctgaccgtggagtccgcgccaggcgagggcagcaccttccgttgctggctgcccgatgctgggcctcttccgggagccgccgatgcctgat BBa_K646000_sequence 1 ctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_K646002_sequence 1 aggagggctgctatgagccgggacccgttgcccttttttccaccgctttaccttggtggcccggaaattaccaccgagaactgcgagcgcgagccgattcatattcccggcagcatccagccgcacggcgccctgctcactgccgacgggcacagcggcgaggtgctccagatgagcctcaacgcggccacttttctgggacaggaacccacagtgctgcgcggacagaccctcgccgcactgctgcccgagcagtggcccgcgctgcaagcggccctgccccccggctgccccgacgccctgcaataccgcgcaacgctggactggcctgccgccgggcacctttcgctgacggtgcaccgggtcggcgagttgctgattctggagttcgagccgacggaggcctgggacagcaccgggccgcacgcgctgcgcaacgcgatgttcgcgctcgaaagtgcccccaacctgcgggcgctggccgaggtggcgacccagacggtccgcgagctgacgggctttgaccgggtgatgctctacaaatttgcccccgacgccaccggcgaagtgattgccgaggcccgccgtgaggggctgcacgcctttctgggccaccgttttcccgcgtcggacattccggcgcaggcccgcgcgctctacacccggcacctgctgcgcctgaccgccgacacccgcgccgccgccgtgccgctcgatcccgtcctcaacccgcagacgaatgcgcccaccccgctgggcggcgccgtgctgcgcgccacctcgcccatgcacatgcagtacctgcggaacatgggcgtcgggtcgagcctgtcggtgtcggtggtggtcggcggccagctctggggcctgatcgcctgccaccaccagacgccctacgtgttgccgcccgacctgcgaaccacgctcgaatacctgggccgcttgctgagcctgcaagttcaggtcaaggaagcggcggacgtggcggcctttcgccagagcctgcgggagcaccacgcgcgggtggccctcgcggcggcgcactcgctctcgccgcacgacaccctcagtgacccggcgcttgacctgctgggcctgatgcgggccgggggcctgattctgcgtttcgagggccgctggcagacgttgggtgaagtgccgcctgccccggcggtggacgcgctgctggcgtggctcgaaacccagccgggcgccctggtccagaccgacgcgctgggccaactgtggcccgccggcgccgatctcgcccccagcgcagcgggcctgctcgccatcagcgtgggcgagggctggtcggagtgcctcgtctggctgcggcccgaactgcggctggaggtcgcctggggcggggccactcctgaccaggcgaaagacgacctcgggccgcgccactcattcgacacctacctcgaagaaaaacgcggctacgccgagccctggcatcccggcgaaatcgaggaggcgcaggatctacgtgacacattgaccggggcgctgggcgagcgcctgagcgtgattcgtgacctcaaccgggcgctcacacagtcgaacgccgagtggcggcagtacggcttcgttatcagccaccacatgcaggagccggtgcggctcatctcgcagttcgccgagttgctgacgcgccagccccgcgcccaggacgggtctccggactctccgcagaccgagcgcatcaccggctttctgctgcgcgaaacgtcgcgcctgcgcagcctgacgcaagacctccacacctacaccgcgctgctctcggcaccgccgccggtgcgccgccccacgccgctgggccgcgtggtggacgatgtgctgcaagacctcgaaccccgcattgccgacaccggagcgagcatcgaggtggcgcccgagttgcccgtcatcgctgccgacgctggcctgctgcgcgacctgctgctgcatctgatcggcaacgcgctgacgtttggtggcccggagccgcgtattgccgtaaggaccgaacggcaaggcgcgggttggtctatcgcggtcagtgaccagggcgctggcatcgcgcccgagtatcaggaacgaatctttctgctgtttcagcggctcggttcgctcgatgaggcgctgggcaacggcctgggcctgccgctgtgccgcaagatcgccgaactgcatggcggcaccctgaccgtggagtccgcgccaggcgagggcagcaccttccgttgctggctgcccgatgctgggcctcttccgggagccgccgatgcctgat BBa_K646003_sequence 1 taatacgactcactatagggagatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z