BBa_K646001 1 AT-Bph Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter) 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z From 2010 Macquarie Team, isolated from Genomic DNA Bacteriophotochrome from Argobacterium tumefaciens (Now Rhizobium radiobacter), that binds to biliverdin to produce a complex that absorbs far red light which catalyses isomerisation to produce a spectral change. false false _822_ 0 10455 9 Not in stock false Contains two internal Pst1 RE sites. false David Dubow annotation2147992 1 RBS range2147992 1 5 10 annotation2147993 1 AT-Bph range2147993 1 16 2227 BBa_K646000 1 HO1 Hemeoxygenase with RBS 2011-09-28T11:00:00Z 2015-05-08T01:12:58Z Http://www.ncbi.nlm.nih.gov/gene/953483 Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _822_ 0 10455 9 In stock true The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation. false Edward Moh annotation2147982 1 RBS range2147982 1 9 14 annotation2147983 1 Heme Oxygenase 1 range2147983 1 22 744 BBa_K646007 1 BBa_K646007 T7 promoter with Heme oxygenase and Rhizobium radiobacter bacteriophytochrome 2011-09-29T11:00:00Z 2015-05-08T01:12:59Z Origin from genomic DNA and 2011 kit plates. This is an assembly of an Rhizobium radiobacter/Agrobacterium tumefaciens isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. Sequence from National Center for Biotechnology. false false _822_ 0 10455 9 Not in stock false None. false David Dubow component2220000 1 BBa_K646001 component2219997 1 BBa_K646004 annotation2219997 1 BBa_K646004 range2219997 1 1 798 annotation2220000 1 BBa_K646001 range2220000 1 807 3035 BBa_K646004 1 BBa_K646004 T7 promoter with Hemeoxygenase 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z BBa_K646000 and BBa_I712074 Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin. false false _822_ 0 10455 9 Not in stock true None. false David Dubow component2152632 1 BBa_I712074 component2152635 1 BBa_K646000 annotation2152635 1 BBa_K646000 range2152635 1 55 798 annotation2152632 1 BBa_I712074 range2152632 1 1 46 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K646000_sequence 1 ctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_K646004_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K646001_sequence 1 gattaggagggctgctatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagcgcgccgtcgctggaggacgcgctttttcggacggcgcaactggtgtcctcgatcagcggtcatgaccgtaccctgatctatgatttcggactggactggagcggccatgtcgtggcggaagcgggaagcggggctttgccgtcttatctcggccttcgctttccggcgggcgacatcccgccgcaggcgcggcagctctataccatcaatcgcctgcggatgattcccgatgtcgattataagcccgtgccgatcagaccggaggtgaatgcggaaacgggagcggtgctcgatatgagcttttcacagcttcgcagcgtatcgccggttcatctcgaatatatgcgcaatatgggcactgccgcttcgatgtcggtgtccatcgtcgtcaatggcgctctctggggcttgattgcctgccaccacgcgacaccgcattcggtatcccttgccgtacgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgccggcaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacggtgcgctggggcggcgatccgcacaagacggtgcaggagagcgggcgcattcatccgcgcaagtcctttgaaatctggaaggaacaattgcgcaacacctcctttccctggtcagagcccgaacttgcggcagcgagggagcttcgcggcgcgatcatcggcattgtcctgcgcaagaccgaggaaatggcggatctgacacgggagttgcagcgcaccaataaggagctggaggctttttcctattccgtttcgcatgacctgcgcgcgccgttccgtcatattgtcgggtttgcgcagttgttgcgggagcgcagcgatgccctggacgagaagtccctgcattatcttcagatgatatcagaggcggcgctgggtgccggcaggttggtggatgatctcctgaatttttcacagcttggccgaacgcaactcaccctgaaaccggtggatatgcagaaggtggtgagcgaagttcgccgctctctttcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggcttcagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagttgaagaggaaaaaattgcctgaaa BBa_K646007_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagaggattaggagggctgctatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagcgcgccgtcgctggaggacgcgctttttcggacggcgcaactggtgtcctcgatcagcggtcatgaccgtaccctgatctatgatttcggactggactggagcggccatgtcgtggcggaagcgggaagcggggctttgccgtcttatctcggccttcgctttccggcgggcgacatcccgccgcaggcgcggcagctctataccatcaatcgcctgcggatgattcccgatgtcgattataagcccgtgccgatcagaccggaggtgaatgcggaaacgggagcggtgctcgatatgagcttttcacagcttcgcagcgtatcgccggttcatctcgaatatatgcgcaatatgggcactgccgcttcgatgtcggtgtccatcgtcgtcaatggcgctctctggggcttgattgcctgccaccacgcgacaccgcattcggtatcccttgccgtacgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgccggcaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacggtgcgctggggcggcgatccgcacaagacggtgcaggagagcgggcgcattcatccgcgcaagtcctttgaaatctggaaggaacaattgcgcaacacctcctttccctggtcagagcccgaacttgcggcagcgagggagcttcgcggcgcgatcatcggcattgtcctgcgcaagaccgaggaaatggcggatctgacacgggagttgcagcgcaccaataaggagctggaggctttttcctattccgtttcgcatgacctgcgcgcgccgttccgtcatattgtcgggtttgcgcagttgttgcgggagcgcagcgatgccctggacgagaagtccctgcattatcttcagatgatatcagaggcggcgctgggtgccggcaggttggtggatgatctcctgaatttttcacagcttggccgaacgcaactcaccctgaaaccggtggatatgcagaaggtggtgagcgaagttcgccgctctctttcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggcttcagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagttgaagaggaaaaaattgcctgaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z