BBa_K646003 1 BBa_K646003 T7 promoter with Hemeoxygenase 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z BBa_K646000 and BBa_I719005 Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin. false false _822_ 0 10455 9 Not in stock true None. false David Dubow component2152628 1 BBa_I719005 component2152631 1 BBa_K646000 annotation2152628 1 BBa_I719005 range2152628 1 1 23 annotation2152631 1 BBa_K646000 range2152631 1 32 775 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K646001 1 AT-Bph Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter) 2011-09-28T11:00:00Z 2015-05-08T01:12:59Z From 2010 Macquarie Team, isolated from Genomic DNA Bacteriophotochrome from Argobacterium tumefaciens (Now Rhizobium radiobacter), that binds to biliverdin to produce a complex that absorbs far red light which catalyses isomerisation to produce a spectral change. false false _822_ 0 10455 9 Not in stock false Contains two internal Pst1 RE sites. false David Dubow annotation2147992 1 RBS range2147992 1 5 10 annotation2147993 1 AT-Bph range2147993 1 16 2227 BBa_K646000 1 HO1 Hemeoxygenase with RBS 2011-09-28T11:00:00Z 2015-05-08T01:12:58Z Http://www.ncbi.nlm.nih.gov/gene/953483 Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration. false false _822_ 0 10455 9 In stock true The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation. false Edward Moh annotation2147983 1 Heme Oxygenase 1 range2147983 1 22 744 annotation2147982 1 RBS range2147982 1 9 14 BBa_K646008 1 BBa_K646008 T7 promoter with Heme oxygenase and Agrobacterium tumefaciens bacteriophytochrome 2011-09-29T11:00:00Z 2015-05-08T01:12:59Z Genomic DNA and 2011 kit plates. This is an assembly of an Rhizobium radiobacter/Agrobacterium tumefaciens isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene under a T7 promoter. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase oxidation of heme in the heme metabolic pathway to produce the biliverdin. Sequence contains a Ribosome Binding Site. Sequence from National Center for Biotechnology. false false _822_ 0 10455 9 Not in stock false None. false David Dubow component2220005 1 BBa_K646003 component2220008 1 BBa_K646001 annotation2220005 1 BBa_K646003 range2220005 1 1 775 annotation2220008 1 BBa_K646001 range2220008 1 784 3012 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K646000_sequence 1 ctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag BBa_K646008_sequence 1 taatacgactcactatagggagatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagtactagaggattaggagggctgctatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagcgcgccgtcgctggaggacgcgctttttcggacggcgcaactggtgtcctcgatcagcggtcatgaccgtaccctgatctatgatttcggactggactggagcggccatgtcgtggcggaagcgggaagcggggctttgccgtcttatctcggccttcgctttccggcgggcgacatcccgccgcaggcgcggcagctctataccatcaatcgcctgcggatgattcccgatgtcgattataagcccgtgccgatcagaccggaggtgaatgcggaaacgggagcggtgctcgatatgagcttttcacagcttcgcagcgtatcgccggttcatctcgaatatatgcgcaatatgggcactgccgcttcgatgtcggtgtccatcgtcgtcaatggcgctctctggggcttgattgcctgccaccacgcgacaccgcattcggtatcccttgccgtacgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgccggcaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacggtgcgctggggcggcgatccgcacaagacggtgcaggagagcgggcgcattcatccgcgcaagtcctttgaaatctggaaggaacaattgcgcaacacctcctttccctggtcagagcccgaacttgcggcagcgagggagcttcgcggcgcgatcatcggcattgtcctgcgcaagaccgaggaaatggcggatctgacacgggagttgcagcgcaccaataaggagctggaggctttttcctattccgtttcgcatgacctgcgcgcgccgttccgtcatattgtcgggtttgcgcagttgttgcgggagcgcagcgatgccctggacgagaagtccctgcattatcttcagatgatatcagaggcggcgctgggtgccggcaggttggtggatgatctcctgaatttttcacagcttggccgaacgcaactcaccctgaaaccggtggatatgcagaaggtggtgagcgaagttcgccgctctctttcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggcttcagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagttgaagaggaaaaaattgcctgaaa BBa_K646001_sequence 1 gattaggagggctgctatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagcgcgccgtcgctggaggacgcgctttttcggacggcgcaactggtgtcctcgatcagcggtcatgaccgtaccctgatctatgatttcggactggactggagcggccatgtcgtggcggaagcgggaagcggggctttgccgtcttatctcggccttcgctttccggcgggcgacatcccgccgcaggcgcggcagctctataccatcaatcgcctgcggatgattcccgatgtcgattataagcccgtgccgatcagaccggaggtgaatgcggaaacgggagcggtgctcgatatgagcttttcacagcttcgcagcgtatcgccggttcatctcgaatatatgcgcaatatgggcactgccgcttcgatgtcggtgtccatcgtcgtcaatggcgctctctggggcttgattgcctgccaccacgcgacaccgcattcggtatcccttgccgtacgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgccggcaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacggtgcgctggggcggcgatccgcacaagacggtgcaggagagcgggcgcattcatccgcgcaagtcctttgaaatctggaaggaacaattgcgcaacacctcctttccctggtcagagcccgaacttgcggcagcgagggagcttcgcggcgcgatcatcggcattgtcctgcgcaagaccgaggaaatggcggatctgacacgggagttgcagcgcaccaataaggagctggaggctttttcctattccgtttcgcatgacctgcgcgcgccgttccgtcatattgtcgggtttgcgcagttgttgcgggagcgcagcgatgccctggacgagaagtccctgcattatcttcagatgatatcagaggcggcgctgggtgccggcaggttggtggatgatctcctgaatttttcacagcttggccgaacgcaactcaccctgaaaccggtggatatgcagaaggtggtgagcgaagttcgccgctctctttcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggcttcagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagttgaagaggaaaaaattgcctgaaa BBa_K646003_sequence 1 taatacgactcactatagggagatactagagctttaagaaggagatatacatatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z