LOCUS       BBa_K648016             1928 bp    DNA     linear       22-Dec-2024
DEFINITION  Fast-Fusion GFP-Xyle Reporter (TEV cleaveage with long linker)
ACCESSION   BBa_K648016
VERSION     BBa_K648016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648008
     misc_feature    836..865
                     /label=BBa_K648006
     misc_feature    874..1791
                     /label=misc
     CDS             874..1791
                     /label=BBa_K648011
     stem_loop       1811..1854
                     /label=stem_loop
     misc_feature    1800..1879
                     /label=BBa_B0010
     terminator      1800..1879
                     /label=BBa_B0010
     stem_loop       1895..1914
                     /label=T7 TE
     misc_feature    1921..1921
                     /label=stop
     polyA_site      1915..1928
                     /label=polya
     misc_feature    1888..1928
                     /label=BBa_B0012
     terminator      1888..1928
                     /label=BBa_B0012
     terminator      1800..1928
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggaga atttgtattt tcagggttac tagagggtga
      841 aaatttgtat tttcaatctg gtggttacta gagaacaaag gtgtaatgcg accgggccat
      901 gtgcagctgc gtgtactgga catgagcaag gccctggaac actacgtcga gttgctgggc
      961 ctgatcgaga tggaccgtga cgaccagggc cgtgtctatc tgaaggcttg gaccgaagtg
     1021 gataagtttt ccctggtgct acgcgaggct gacgagccgg gcatggattt tatgggtttc
     1081 aaggttgtgg atgaggatgc tctccggcaa ctggagcggg atctgatggc atatggctgt
     1141 gccgttgagc agctacccgc aggtgaactg aacagttgtg gccgccgcgt gcgcttccag
     1201 gccccctccg ggcatcactt cgagttgtat gcagacaagg aatatactgg aaagtggggt
     1261 ttgaatgacg tcaatcccga ggcatggccg cgcgatctga aaggtatggc ggctgtgcgt
     1321 ttcgaccacg ccctcatgta tggcgacgaa ttgccagcga cctatgacct gttcaccaag
     1381 gtgctcggtt tctatctggc cgaacaggtg ctggacgaaa atggcacgcg cgtcgcccag
     1441 tttctcagtc tgtcgaccaa ggcccacgac gtggccttca ttcaccatcc ggaaaaaggc
     1501 cgcctccatc atgtgtcctt ccacctcgaa acctgggaag acttgcttcg cgccgccgac
     1561 ctgatctcca tgaccgacac atctatcgat atcggcccaa cccgccacgg cctcactcac
     1621 ggcaagacca tctacttctt cgacccgtcc ggtaaccgca acgaagtgtt ctgcggggga
     1681 gattacaact acccggacca caaaccagtg acctggacca ccgaccagct gggcaaggcg
     1741 atcttttacc acgaccgcat tctcaacgaa cgattcatga ccgtgctgac ctactagagc
     1801 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1861 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1921 cgtttata
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