LOCUS       BBa_K648017             1220 bp    DNA     linear       23-May-2024
DEFINITION  Fast-Fusion GFP-LacZa Reporter (TEV cleavage with small linker)
ACCESSION   BBa_K648017
VERSION     BBa_K648017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648008
     misc_feature    836..847
                     /label=BBa_K648005
     CDS             856..1083
                     /label=protein
     CDS             856..1083
                     /label=BBa_K648012
     stem_loop       1103..1146
                     /label=stem_loop
     misc_feature    1092..1171
                     /label=BBa_B0010
     terminator      1092..1171
                     /label=BBa_B0010
     stem_loop       1187..1206
                     /label=T7 TE
     misc_feature    1213..1213
                     /label=stop
     polyA_site      1207..1220
                     /label=polya
     misc_feature    1180..1220
                     /label=BBa_B0012
     terminator      1180..1220
                     /label=BBa_B0012
     terminator      1092..1220
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggaga atttgtattt tcagggttac tagagggtgg
      841 ttctggttac tagagaccat gattacggat tcactggccg tcgttttaca acgtcgtgac
      901 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      961 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1021 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
     1081 tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1141 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1201 tgggcctttc tgcgtttata
//