BBa_K648019
type: ComponentDefinition
persistent identity: BBa K648019
display id: BBa_K648019
version: 1
was generated by: iGEM to SBOL conversion
description: Fast-Fusion GFP-LacZa Reporter (TEV cleavage with long linker)
created: 2011-07-04T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: The design of this part was inspired by the Imperial College London 2010 iGEM Team's Fast-Response module, part BBa_K316007.
owned by: james
owned by: myers
mutable description: This is a fast-acting reporter using the LacZa gene (BBa_K648012) fused to GFP (BBa_K648013). In its normal un-cleaved state the polymerization ability necessary for enzymatic action is inactivated. Once the fusion protein is cleaved by the TEV protease the B-gal enzyme encoded by the LacZa gene is able to convert X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole, the latter of which is oxidized to the dark blue product 5,5'-dibromo-4,4'-dichloro-indigo. This reporter is able to produce a visible blue color response in a matter of minutes. This version uses the TEV cleavage site (BBa_K648008) followed by the smallest of the three flexible fusion protein linkers (BBa_K648005). The linker is attached to the N-terminus of the LacZa gene.
discontinued: false
dominant: false
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: All parts were assembled via standard 25 assembly methods
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K648012
component: BBa_J23100
component: BBa_K648006
component: BBa_K648008
component: BBa_K648013
component: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K648013
sequence annotation: BBa_K648008
sequence annotation: BBa_K648006
sequence annotation: BBa_K648012
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K648019_sequence/1

BBa_B0015
type: Component
persistent identity: component2122998
display id: component2122998
version: 1
top level: BBa_K648019
definition: BBa_B0015
access: public

BBa_K648012
type: Component
persistent identity: component2122991
display id: component2122991
version: 1
top level: BBa_K648019
definition: BBa_K648012
access: public

BBa_J23100
type: Component
persistent identity: component2122983
display id: component2122983
version: 1
top level: BBa_K648019
definition: BBa_J23100
access: public

BBa_K648006
type: Component
persistent identity: component2122989
display id: component2122989
version: 1
top level: BBa_K648019
definition: BBa_K648006
access: public

BBa_K648008
type: Component
persistent identity: component2122988
display id: component2122988
version: 1
top level: BBa_K648019
definition: BBa_K648008
access: public

BBa_K648013
type: Component
persistent identity: component2122987
display id: component2122987
version: 1
top level: BBa_K648019
definition: BBa_K648013
access: public

BBa_B0034
type: Component
persistent identity: component2122985
display id: component2122985
version: 1
top level: BBa_K648019
definition: BBa_B0034
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2122983
display id: annotation2122983
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122983/range2122983/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K648019/annotation2122983/range2122983/1
type: Range
persistent identity: range2122983
display id: range2122983
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2122985
display id: annotation2122985
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122985/range2122985/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K648019/annotation2122985/range2122985/1
type: Range
persistent identity: range2122985
display id: range2122985
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K648013
type: SequenceAnnotation
persistent identity: annotation2122987
display id: annotation2122987
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122987/range2122987/1
component: BBa_K648013

https://synbiohub.org/public/igem/BBa_K648019/annotation2122987/range2122987/1
type: Range
persistent identity: range2122987
display id: range2122987
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 64
end: 798
orientation: inline

BBa_K648008
type: SequenceAnnotation
persistent identity: annotation2122988
display id: annotation2122988
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122988/range2122988/1
component: BBa_K648008

https://synbiohub.org/public/igem/BBa_K648019/annotation2122988/range2122988/1
type: Range
persistent identity: range2122988
display id: range2122988
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 807
end: 827
orientation: inline

BBa_K648006
type: SequenceAnnotation
persistent identity: annotation2122989
display id: annotation2122989
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122989/range2122989/1
component: BBa_K648006

https://synbiohub.org/public/igem/BBa_K648019/annotation2122989/range2122989/1
type: Range
persistent identity: range2122989
display id: range2122989
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 836
end: 865
orientation: inline

BBa_K648012
type: SequenceAnnotation
persistent identity: annotation2122991
display id: annotation2122991
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122991/range2122991/1
component: BBa_K648012

https://synbiohub.org/public/igem/BBa_K648019/annotation2122991/range2122991/1
type: Range
persistent identity: range2122991
display id: range2122991
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 874
end: 1101
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2122998
display id: annotation2122998
version: 1
top level: BBa_K648019
location: https://synbiohub.org/public/igem/BBa_K648019/annotation2122998/range2122998/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K648019/annotation2122998/range2122998/1
type: Range
persistent identity: range2122998
display id: range2122998
version: 1
top level: BBa_K648019
direction: direction/reverse
start: 1110
end: 1238
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K648006
type: ComponentDefinition
persistent identity: BBa K648006
display id: BBa_K648006
version: 1
was generated by: iGEM to SBOL conversion
description: Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
created: 2011-07-02T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z) Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529
owned by: james
owned by: myers
mutable description: This is the longest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the ten amino acids GENLYFQSGG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward: TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtgaaaatttgtattttcaa Reverse: AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccaccagattgaaaatacaaattttcacc
star: false
creator: Jim Rose, Alex Bina, Ben Alouidor, Brian Avison
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K648006_sequence/1

BBa_K648008
type: ComponentDefinition
persistent identity: BBa K648008
display id: BBa_K648008
version: 1
was generated by: iGEM to SBOL conversion
description: TEV protease cleaveage site with Standard 25 Prefix/Suffix
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: Phan, J., Zdanov, A., Evdokimov, A. G., Tropea, J. E., Peters, H. P. K., Kapust, R. B., Li, M., Wlodawer, A., and Waugh, D. S. (2002). Structural basis for the substrate specificity of tobacco etch virus protease. J. Biol. Chem. 277: 50564-50572.
owned by: james
owned by: myers
mutable description: This is the cleaveage site for th Tobacco etch virus(TEV) protease commonly used for cleaving fusion proteins. It encodes for the amino acids E N L Y F Q G which are cleaved by the TEV protease. This part also contains the prefix/suffix required for standard 25 assembly into fusion parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward 5'---ATTAGAATTCGCGGCCGCTTCTAGATGGCCGGCGAGAATTTGTATTTTCAGGG---3' Reverse 5'---TAATCTGCAGCGGCCGCTACTAGTATTAACCGGTACCCTGAAAATACAAATTCTC---3'
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K648008_sequence/1

BBa_K648012
type: ComponentDefinition
persistent identity: BBa K648012
display id: BBa_K648012
version: 1
was generated by: iGEM to SBOL conversion
description: LacZa wtih Standard 25 Prefix/Suffix
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: This part was amplified out of registry stock of LacZa in order to include the standard 25 prefix/suffix
owned by: james
owned by: myers
mutable description: This is the lacZa subunit used to complement cells for blue/white screening. It has been cloned into a vector including the prefix/suffix required for standard 25 assembly for fusion proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCACCATGATTACGGATTCAC (57.74 oC) Reverse ATAACTGCAGCGGCCGCTACTAGTATTAACCGGTTCACTCCAGCCAGC (55.05 oC)
star: false
creator: Jim Rose
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K648012_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2122826
display id: annotation2122826
version: 1
top level: BBa_K648012
location: https://synbiohub.org/public/igem/BBa_K648012/annotation2122826/range2122826/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K648012/annotation2122826/range2122826/1
type: Range
persistent identity: range2122826
display id: range2122826
version: 1
top level: BBa_K648012
direction: direction/forward
start: 1
end: 228
orientation: inline

BBa_K648013
type: ComponentDefinition
persistent identity: BBa K648013
display id: BBa_K648013
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with Standard 25 Prefix/Suffix
created: 2011-07-03T11:00:00Z
modified: 2016-01-25T02:32:21Z
mutable provenance: This part is similar to the part BBa_E0040 commonly used as a reporter.
owned by: james
owned by: myers
mutable description: This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
discontinued: false
dominant: false
group u list: _825_
m user id: 4206
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward (56.88 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC Reverse (56.95 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648013_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122827
display id: annotation2122827
version: 1
top level: BBa_K648013
location: https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
type: Range
persistent identity: range2122827
display id: range2122827
version: 1
top level: BBa_K648013
direction: direction/forward
start: 1
end: 735
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K648019_sequence/1
type: Sequence
persistent identity: BBa K648019 sequence
display id: BBa_K648019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaagtactagaggagaatttgtattttcagggttactagagggtgaaaatttgtattttcaatctggtggttactagagaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648006_sequence/1
type: Sequence
persistent identity: BBa K648006 sequence
display id: BBa_K648006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgaaaatttgtattttcaatctggtggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648008_sequence/1
type: Sequence
persistent identity: BBa K648008 sequence
display id: BBa_K648008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagaatttgtattttcagggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648012_sequence/1
type: Sequence
persistent identity: BBa K648012 sequence
display id: BBa_K648012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648013_sequence/1
type: Sequence
persistent identity: BBa K648013 sequence
display id: BBa_K648013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z