LOCUS       BBa_K648020             1910 bp    DNA     linear       26-Dec-2024
DEFINITION  Fast-Fusion GFP-Xyle Reporter (RecA cleaveage with small linker)
ACCESSION   BBa_K648020
VERSION     BBa_K648020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648009
     misc_feature    836..847
                     /label=BBa_K648005
     misc_feature    856..1773
                     /label=misc
     CDS             856..1773
                     /label=BBa_K648011
     stem_loop       1793..1836
                     /label=stem_loop
     misc_feature    1782..1861
                     /label=BBa_B0010
     terminator      1782..1861
                     /label=BBa_B0010
     stem_loop       1877..1896
                     /label=T7 TE
     misc_feature    1903..1903
                     /label=stop
     polyA_site      1897..1910
                     /label=polya
     misc_feature    1870..1910
                     /label=BBa_B0012
     terminator      1870..1910
                     /label=BBa_B0012
     terminator      1782..1910
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggttc aggcagggat gttctcatac tagagggtgg
      841 ttctggttac tagagaacaa aggtgtaatg cgaccgggcc atgtgcagct gcgtgtactg
      901 gacatgagca aggccctgga acactacgtc gagttgctgg gcctgatcga gatggaccgt
      961 gacgaccagg gccgtgtcta tctgaaggct tggaccgaag tggataagtt ttccctggtg
     1021 ctacgcgagg ctgacgagcc gggcatggat tttatgggtt tcaaggttgt ggatgaggat
     1081 gctctccggc aactggagcg ggatctgatg gcatatggct gtgccgttga gcagctaccc
     1141 gcaggtgaac tgaacagttg tggccgccgc gtgcgcttcc aggccccctc cgggcatcac
     1201 ttcgagttgt atgcagacaa ggaatatact ggaaagtggg gtttgaatga cgtcaatccc
     1261 gaggcatggc cgcgcgatct gaaaggtatg gcggctgtgc gtttcgacca cgccctcatg
     1321 tatggcgacg aattgccagc gacctatgac ctgttcacca aggtgctcgg tttctatctg
     1381 gccgaacagg tgctggacga aaatggcacg cgcgtcgccc agtttctcag tctgtcgacc
     1441 aaggcccacg acgtggcctt cattcaccat ccggaaaaag gccgcctcca tcatgtgtcc
     1501 ttccacctcg aaacctggga agacttgctt cgcgccgccg acctgatctc catgaccgac
     1561 acatctatcg atatcggccc aacccgccac ggcctcactc acggcaagac catctacttc
     1621 ttcgacccgt ccggtaaccg caacgaagtg ttctgcgggg gagattacaa ctacccggac
     1681 cacaaaccag tgacctggac caccgaccag ctgggcaagg cgatctttta ccacgaccgc
     1741 attctcaacg aacgattcat gaccgtgctg acctactaga gccaggcatc aaataaaacg
     1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//