BBa_K648020
type: ComponentDefinition
persistent identity: BBa K648020
display id: BBa_K648020
version: 1
was generated by: iGEM to SBOL conversion
description: Fast-Fusion GFP-Xyle Reporter (RecA cleaveage with small linker)
created: 2011-07-04T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: The design of this part was inspired by the Imperial College London 2010 iGEM Team's Fast-Response module, part BBa_K316007.
owned by: james
owned by: myers
mutable description: This is a fast-acting reporter using the Xyle gene (BBa_K648011) fused to GFP. In its normal un-cleaved state the polymerization ability necessary for enzymatic action is inactivated. Once the fusion protein is cleaved by the RecA, which is activated by damaged DNA, the catechol-2,3-dioxygenase enzyme encoded by the Xyle gene is able to convert catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This reporter is able to produce a visible color response in a matter of minutes. This version uses the RecA cleavage site (BBa_K648009) followed by the smallest of the three flexible fusion protein linkers (BBa_K648005). The linker is attached to the N-terminus of the Xyle gene.
discontinued: false
dominant: false
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: All parts were assembled via standard 25 assembly methods.
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K648011
component: BBa_K648005
component: BBa_K648009
component: BBa_K648013
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K648013
sequence annotation: BBa_K648009
sequence annotation: BBa_K648005
sequence annotation: BBa_K648011
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K648020_sequence/1

BBa_B0015
type: Component
persistent identity: component2123014
display id: component2123014
version: 1
top level: BBa_K648020
definition: BBa_B0015
access: public

BBa_K648011
type: Component
persistent identity: component2123007
display id: component2123007
version: 1
top level: BBa_K648020
definition: BBa_K648011
access: public

BBa_K648005
type: Component
persistent identity: component2123005
display id: component2123005
version: 1
top level: BBa_K648020
definition: BBa_K648005
access: public

BBa_K648009
type: Component
persistent identity: component2123004
display id: component2123004
version: 1
top level: BBa_K648020
definition: BBa_K648009
access: public

BBa_K648013
type: Component
persistent identity: component2123003
display id: component2123003
version: 1
top level: BBa_K648020
definition: BBa_K648013
access: public

BBa_B0034
type: Component
persistent identity: component2123001
display id: component2123001
version: 1
top level: BBa_K648020
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2122999
display id: component2122999
version: 1
top level: BBa_K648020
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2122999
display id: annotation2122999
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2122999/range2122999/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K648020/annotation2122999/range2122999/1
type: Range
persistent identity: range2122999
display id: range2122999
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2123001
display id: annotation2123001
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123001/range2123001/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K648020/annotation2123001/range2123001/1
type: Range
persistent identity: range2123001
display id: range2123001
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K648013
type: SequenceAnnotation
persistent identity: annotation2123003
display id: annotation2123003
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123003/range2123003/1
component: BBa_K648013

https://synbiohub.org/public/igem/BBa_K648020/annotation2123003/range2123003/1
type: Range
persistent identity: range2123003
display id: range2123003
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 64
end: 798
orientation: inline

BBa_K648009
type: SequenceAnnotation
persistent identity: annotation2123004
display id: annotation2123004
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123004/range2123004/1
component: BBa_K648009

https://synbiohub.org/public/igem/BBa_K648020/annotation2123004/range2123004/1
type: Range
persistent identity: range2123004
display id: range2123004
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 807
end: 827
orientation: inline

BBa_K648005
type: SequenceAnnotation
persistent identity: annotation2123005
display id: annotation2123005
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123005/range2123005/1
component: BBa_K648005

https://synbiohub.org/public/igem/BBa_K648020/annotation2123005/range2123005/1
type: Range
persistent identity: range2123005
display id: range2123005
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 836
end: 847
orientation: inline

BBa_K648011
type: SequenceAnnotation
persistent identity: annotation2123007
display id: annotation2123007
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123007/range2123007/1
component: BBa_K648011

https://synbiohub.org/public/igem/BBa_K648020/annotation2123007/range2123007/1
type: Range
persistent identity: range2123007
display id: range2123007
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 856
end: 1773
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2123014
display id: annotation2123014
version: 1
top level: BBa_K648020
location: https://synbiohub.org/public/igem/BBa_K648020/annotation2123014/range2123014/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K648020/annotation2123014/range2123014/1
type: Range
persistent identity: range2123014
display id: range2123014
version: 1
top level: BBa_K648020
direction: direction/reverse
start: 1782
end: 1910
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K648005
type: ComponentDefinition
persistent identity: BBa K648005
display id: BBa_K648005
version: 1
was generated by: iGEM to SBOL conversion
description: Short Fusion Protein Linker: GGSG with standard 25 prefix/suffix
created: 2011-07-02T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z) Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529
owned by: james
owned by: myers
mutable description: This is the shortest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the four amino acids GGSG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward (57.78 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtggttctggtACC Reverse (57.78 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccagaaccaccG
star: false
creator: Jim Rose, Alex Bina, Ben Alouidor, Brian Avison
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K648005_sequence/1

BBa_K648009
type: ComponentDefinition
persistent identity: BBa K648009
display id: BBa_K648009
version: 1
was generated by: iGEM to SBOL conversion
description: RecA Cleavage Site with Standard 25 Prefix/Suffix
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: Kim, B, and Little, LB. "LexA and lambda Cl repressors as enzymes: specific cleavage in an intermolecular reaction.." Cell 73.6 (1993): 1165-73. Web. 4 Jul 2011. <http://www.ncbi.nlm.nih.gov/pubmed/8513500>.
owned by: james
owned by: myers
mutable description: This part encodes for the amino acids V Q A G M F S which are cleavage by the RecA protein within the cell. This sequence was taken from the CI repressor protein which is also cleaved by RecA.
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward Primer 5'---ATTAGAATTCGCGGCCGCTTCTAGATGGCCGGCgttCAGGCAGGGATGTTC---3??? Reverse Primer 5???---TAATCTGCAGCGGCCGCTACTAGTATTAACCGGTtgagaacatccctgcctg ---3???
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K648009_sequence/1

BBa_K648011
type: ComponentDefinition
persistent identity: BBa K648011
display id: BBa_K648011
version: 1
was generated by: iGEM to SBOL conversion
description: Standard 25-Ready Xyle Reporter
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
mutable provenance: The original genetic material for this part came from part BBa_K316007 which was mutated via PCR based site-directed mutagensis. The xyle portion of this large part was then amplified via PCR reaction and cloned into a new vector along with a prefix/suffix for standard 25 assembly.
owned by: james
owned by: myers
mutable description: This is a mutated version of the Xyle reporter gene which encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This version of Xyle has been made compatible with standard 25 assembly methods by removing three restriction sites (two NgoMIV sites: at bp 315 and 486, as well as one AgeI site: at bp 837). These mutations were made synonymous with the original sequence and codon optimized for E. Coli. Because of the synonymous mutations, this gene can be easily used to create fusion protein parts. For more information on the Xyle gene and its uses as a reporter see part BBa_J33204.
discontinued: false
dominant: false
experience: experience/Works
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The mutations in this gene were made so that they were synonymous and codon optimized for E. coli cells. The part also contains the prefix/suffix for standard 25 assembly with addition restriction sites NgoMIV and AgeI.
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648011_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122825
display id: annotation2122825
version: 1
top level: BBa_K648011
location: https://synbiohub.org/public/igem/BBa_K648011/annotation2122825/range2122825/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K648011/annotation2122825/range2122825/1
type: Range
persistent identity: range2122825
display id: range2122825
version: 1
top level: BBa_K648011
direction: direction/forward
start: 1
end: 918
orientation: inline

BBa_K648013
type: ComponentDefinition
persistent identity: BBa K648013
display id: BBa_K648013
version: 1
was generated by: iGEM to SBOL conversion
description: GFP with Standard 25 Prefix/Suffix
created: 2011-07-03T11:00:00Z
modified: 2016-01-25T02:32:21Z
mutable provenance: This part is similar to the part BBa_E0040 commonly used as a reporter.
owned by: james
owned by: myers
mutable description: This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
discontinued: false
dominant: false
group u list: _825_
m user id: 4206
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward (56.88 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC Reverse (56.95 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
star: false
creator: Jim Rose
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648013_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122827
display id: annotation2122827
version: 1
top level: BBa_K648013
location: https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
type: Range
persistent identity: range2122827
display id: range2122827
version: 1
top level: BBa_K648013
direction: direction/forward
start: 1
end: 735
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K648020_sequence/1
type: Sequence
persistent identity: BBa K648020 sequence
display id: BBa_K648020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaagtactagaggttcaggcagggatgttctcatactagagggtggttctggttactagagaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccgccgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccagcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccagtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648005_sequence/1
type: Sequence
persistent identity: BBa K648005 sequence
display id: BBa_K648005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648009_sequence/1
type: Sequence
persistent identity: BBa K648009 sequence
display id: BBa_K648009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gttcaggcagggatgttctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648011_sequence/1
type: Sequence
persistent identity: BBa K648011 sequence
display id: BBa_K648011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccgccgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccagcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccagtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K648013_sequence/1
type: Sequence
persistent identity: BBa K648013 sequence
display id: BBa_K648013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaagattataaagatgatgacgacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z