LOCUS       BBa_K648021             1916 bp    DNA     linear       22-Dec-2024
DEFINITION  Fast-Fusion GFP-Xyle Reporter (RecA cleaveage with Medium linker)
ACCESSION   BBa_K648021
VERSION     BBa_K648021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648009
     misc_feature    836..853
                     /label=BBa_K648007
     misc_feature    862..1779
                     /label=misc
     CDS             862..1779
                     /label=BBa_K648011
     stem_loop       1799..1842
                     /label=stem_loop
     misc_feature    1788..1867
                     /label=BBa_B0010
     terminator      1788..1867
                     /label=BBa_B0010
     stem_loop       1883..1902
                     /label=T7 TE
     misc_feature    1909..1909
                     /label=stop
     polyA_site      1903..1916
                     /label=polya
     misc_feature    1876..1916
                     /label=BBa_B0012
     terminator      1876..1916
                     /label=BBa_B0012
     terminator      1788..1916
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggttc aggcagggat gttctcatac tagagggagg
      841 ttcaggaggc agctactaga gaacaaaggt gtaatgcgac cgggccatgt gcagctgcgt
      901 gtactggaca tgagcaaggc cctggaacac tacgtcgagt tgctgggcct gatcgagatg
      961 gaccgtgacg accagggccg tgtctatctg aaggcttgga ccgaagtgga taagttttcc
     1021 ctggtgctac gcgaggctga cgagccgggc atggatttta tgggtttcaa ggttgtggat
     1081 gaggatgctc tccggcaact ggagcgggat ctgatggcat atggctgtgc cgttgagcag
     1141 ctacccgcag gtgaactgaa cagttgtggc cgccgcgtgc gcttccaggc cccctccggg
     1201 catcacttcg agttgtatgc agacaaggaa tatactggaa agtggggttt gaatgacgtc
     1261 aatcccgagg catggccgcg cgatctgaaa ggtatggcgg ctgtgcgttt cgaccacgcc
     1321 ctcatgtatg gcgacgaatt gccagcgacc tatgacctgt tcaccaaggt gctcggtttc
     1381 tatctggccg aacaggtgct ggacgaaaat ggcacgcgcg tcgcccagtt tctcagtctg
     1441 tcgaccaagg cccacgacgt ggccttcatt caccatccgg aaaaaggccg cctccatcat
     1501 gtgtccttcc acctcgaaac ctgggaagac ttgcttcgcg ccgccgacct gatctccatg
     1561 accgacacat ctatcgatat cggcccaacc cgccacggcc tcactcacgg caagaccatc
     1621 tacttcttcg acccgtccgg taaccgcaac gaagtgttct gcgggggaga ttacaactac
     1681 ccggaccaca aaccagtgac ctggaccacc gaccagctgg gcaaggcgat cttttaccac
     1741 gaccgcattc tcaacgaacg attcatgacc gtgctgacct actagagcca ggcatcaaat
     1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//