LOCUS       BBa_K648024             1226 bp    DNA     linear       22-Dec-2024
DEFINITION  Fast-Fusion GFP-LacZa Reporter (RecA cleavage with Medium linker)
ACCESSION   BBa_K648024
VERSION     BBa_K648024.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648009
     misc_feature    836..853
                     /label=BBa_K648007
     CDS             862..1089
                     /label=protein
     CDS             862..1089
                     /label=BBa_K648012
     stem_loop       1109..1152
                     /label=stem_loop
     misc_feature    1098..1177
                     /label=BBa_B0010
     terminator      1098..1177
                     /label=BBa_B0010
     stem_loop       1193..1212
                     /label=T7 TE
     misc_feature    1219..1219
                     /label=stop
     polyA_site      1213..1226
                     /label=polya
     misc_feature    1186..1226
                     /label=BBa_B0012
     terminator      1186..1226
                     /label=BBa_B0012
     terminator      1098..1226
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggttc aggcagggat gttctcatac tagagggagg
      841 ttcaggaggc agctactaga gaccatgatt acggattcac tggccgtcgt tttacaacgt
      901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1021 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
     1081 ctggagtgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttata
//