LOCUS       BBa_K648025             1238 bp    DNA     linear       25-Nov-2024
DEFINITION  Fast-Fusion GFP-LacZa Reporter (RecA cleavage with Long linker)
ACCESSION   BBa_K648025
VERSION     BBa_K648025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..798
                     /label=misc
     CDS             64..798
                     /label=BBa_K648013
     misc_feature    807..827
                     /label=BBa_K648009
     misc_feature    836..865
                     /label=BBa_K648006
     CDS             874..1101
                     /label=protein
     CDS             874..1101
                     /label=BBa_K648012
     stem_loop       1121..1164
                     /label=stem_loop
     misc_feature    1110..1189
                     /label=BBa_B0010
     terminator      1110..1189
                     /label=BBa_B0010
     stem_loop       1205..1224
                     /label=T7 TE
     misc_feature    1231..1231
                     /label=stop
     polyA_site      1225..1238
                     /label=polya
     misc_feature    1198..1238
                     /label=BBa_B0012
     terminator      1198..1238
                     /label=BBa_B0012
     terminator      1110..1238
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gagcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaagattat
      781 aaagatgatg acgacaagta ctagaggttc aggcagggat gttctcatac tagagggtga
      841 aaatttgtat tttcaatctg gtggttacta gagaccatga ttacggattc actggccgtc
      901 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      961 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1021 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
     1081 ccggaaagct ggctggagtg atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1201 cactggctca ccttcgggtg ggcctttctg cgtttata
//