##gff-version 3
##sequence-region BBa_K648105 237 861
BBa_K648105	.	sequence_alteration	783	783	.	+	0	ID=annotation2160244;Name=EcoRI mutation
BBa_K648105	.	sequence_alteration	237	237	.	+	0	ID=annotation2160243;Name=PstI mutation
BBa_K648105	.	sequence_alteration	861	861	.	+	0	ID=annotation2160245;Name=Lys286 mutation
>BBa_K648105
atggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctcca
tcatgcgcctgggtgaagaccgttccatggatgtggaaaccatctctaccggttcgctttcactggatatcgcgcttgg
ggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaaccacgctgacgctgcaa
gtgatcgccgcagcgcagcgtgaaggtaaaacctgtgcgtttatcgatgctgaacacgcgctggacccaatctacgcac
gtaaactgggcgtcgatatcgacaacctgctgtgctcccagccggacaccggcgagcaggcactggaaatctgtgacgc
cctggcgcgttctggcgcagtagacgttatcgtcgttgactccgtggcggcactgacgccgaaagcggaaatcgaaggc
gaaatcgacgactctcacatgggccttgcggcacgtatgatgagccaggcgatgcataagctggcgggtaacctgaagc
agtccaacacgctgctgatcttcatcaaccagatccgtatgaaaattggtgtgatgttcggtaacccggaaaccactac
cggtggtaacgcgctgaaattctacgcctctgttcgtctcgacatccgtcgtatcggcgcggtgaaagagggcgaaaac
gtggtgggtagcgaaacccgcgtgaaagtggtgaagaacaaaatcgctgcgccgtttaaacaggctgaatttcagatcc
tctacggcgaaggtatcaacttctacggcgaactggttgacctgggcgtaaaagagaagctgatcgagaacgcaggcgc
gtggtacagctacaaaggtgagaagatcggtcagggtaaagcgaatgcgactgcctggctgaaagataacccggaaacc
gcgaaagagatcgagaagaaagtacgtgagttgctgctgagcaacccgaactcaacgccggatttctctgtagatgata
gcgaaggcgtagcagaaactaacgaagatttttaa