##gff-version 3
##sequence-region BBa_K648106 237 783
BBa_K648106	.	sequence_alteration	237	237	.	+	0	ID=annotation2160283;Name=PstI mutation
BBa_K648106	.	sequence_alteration	783	783	.	+	0	ID=annotation2160284;Name=EcoRI mutation
BBa_K648106	.	sequence_alteration	731	731	.	+	0	ID=annotation2160285;Name=Arg243
>BBa_K648106
atggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctcca
tcatgcgcctgggtgaagaccgttccatggatgtggaaaccatctctaccggttcgctttcactggatatcgcgcttgg
ggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaaccacgctgacgctgcaa
gtgatcgccgcagcgcagcgtgaaggtaaaacctgtgcgtttatcgatgctgaacacgcgctggacccaatctacgcac
gtaaactgggcgtcgatatcgacaacctgctgtgctcccagccggacaccggcgagcaggcactggaaatctgtgacgc
cctggcgcgttctggcgcagtagacgttatcgtcgttgactccgtggcggcactgacgccgaaagcggaaatcgaaggc
gaaatcgacgactctcacatgggccttgcggcacgtatgatgagccaggcgatgcataagctggcgggtaacctgaagc
agtccaacacgctgctgatcttcatcaaccagatccgtatgaaaattggtgtgatgttcggtaacccggaaaccactac
cggtggtaacgcgctgaaattctacgcctctgttcgtctcgacatccgtcgtatcggcgcggtgaaagagggcgaaaac
gtggtgggtagcgaaacccacgtgaaagtggtgaagaacaaaatcgctgcgccgtttaaacaggctgaatttcagatcc
tctacggcgaaggtatcaacttctacggcgaactggttgacctgggcgtaaaagagaagctgatcgagaaagcaggcgc
gtggtacagctacaaaggtgagaagatcggtcagggtaaagcgaatgcgactgcctggctgaaagataacccggaaacc
gcgaaagagatcgagaagaaagtacgtgagttgctgctgagcaacccgaactcaacgccggatttctctgtagatgata
gcgaaggcgtagcagaaactaacgaagatttttaa