##gff-version 3
##sequence-region BBa_K648107 237 861
BBa_K648107	.	sequence_alteration	237	237	.	+	0	ID=annotation2160286;Name=PstI mutation
BBa_K648107	.	sequence_alteration	861	861	.	+	0	ID=annotation2160288;Name=Lys286
BBa_K648107	.	sequence_alteration	783	783	.	+	0	ID=annotation2160287;Name=EcoRI mutation
BBa_K648107	.	sequence_alteration	731	731	.	+	0	ID=annotation2160289;Name=Arg243
>BBa_K648107
atggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctcca
tcatgcgcctgggtgaagaccgttccatggatgtggaaaccatctctaccggttcgctttcactggatatcgcgcttgg
ggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaaccacgctgacgctgcaa
gtgatcgccgcagcgcagcgtgaaggtaaaacctgtgcgtttatcgatgctgaacacgcgctggacccaatctacgcac
gtaaactgggcgtcgatatcgacaacctgctgtgctcccagccggacaccggcgagcaggcactggaaatctgtgacgc
cctggcgcgttctggcgcagtagacgttatcgtcgttgactccgtggcggcactgacgccgaaagcggaaatcgaaggc
gaaatcgacgactctcacatgggccttgcggcacgtatgatgagccaggcgatgcataagctggcgggtaacctgaagc
agtccaacacgctgctgatcttcatcaaccagatccgtatgaaaattggtgtgatgttcggtaacccggaaaccactac
cggtggtaacgcgctgaaattctacgcctctgttcgtctcgacatccgtcgtatcggcgcggtgaaagagggcgaaaac
gtggtgggtagcgaaacccacgtgaaagtggtgaagaacaaaatcgctgcgccgtttaaacaggctgaatttcagatcc
tctacggcgaaggtatcaacttctacggcgaactggttgacctgggcgtaaaagagaagctgatcgagaacgcaggcgc
gtggtacagctacaaaggtgagaagatcggtcagggtaaagcgaatgcgactgcctggctgaaagataacccggaaacc
gcgaaagagatcgagaagaaagtacgtgagttgctgctgagcaacccgaactcaacgccggatttctctgtagatgata
gcgaaggcgtagcagaaactaacgaagatttttaa