##gff-version 3
##sequence-region BBa_K648108 237 861
BBa_K648108	.	sequence_alteration	530	530	.	+	0	ID=annotation2160313;Name=RecA activation mutation
BBa_K648108	.	sequence_alteration	783	783	.	+	0	ID=annotation2160310;Name=EcoRI mutation
BBa_K648108	.	sequence_alteration	861	861	.	+	0	ID=annotation2160311;Name=Lys286 mutation
BBa_K648108	.	sequence_alteration	237	237	.	+	0	ID=annotation2160309;Name=PstI mutation
BBa_K648108	.	sequence_alteration	731	731	.	+	0	ID=annotation2160312;Name=Arg243 mutation
>BBa_K648108
atggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctcca
tcatgcgcctgggtgaagaccgttccatggatgtggaaaccatctctaccggttcgctttcactggatatcgcgcttgg
ggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaaccacgctgacgctgcaa
gtgatcgccgcagcgcagcgtgaaggtaaaacctgtgcgtttatcgatgctgaacacgcgctggacccaatctacgcac
gtaaactgggcgtcgatatcgacaacctgctgtgctcccagccggacaccggcgagcaggcactggaaatctgtgacgc
cctggcgcgttctggcgcagtagacgttatcgtcgttgactccgtggcggcactgacgccgaaagcggaaatcgaaggc
gaaatcgacgactctcacatgggccttgcggcacgtatgatgagccaggcgatgcgtaagctggcgggtaacctgaagc
agtccaacacgctgctgatcttcatcaaccagatccgtatgaaaattggtgtgatgttcggtaacccggaaaccactac
cggtggtaacgcgctgaaattctacgcctctgttcgtctcgacatccgtcgtatcggcgcggtgaaagagggcgaaaac
gtggtgggtagcgaaacccacgtgaaagtggtgaagaacaaaatcgctgcgccgtttaaacaggctgaatttcagatcc
tctacggcgaaggtatcaacttctacggcgaactggttgacctgggcgtaaaagagaagctgatcgagaacgcaggcgc
gtggtacagctacaaaggtgagaagatcggtcagggtaaagcgaatgcgactgcctggctgaaagataacccggaaacc
gcgaaagagatcgagaagaaagtacgtgagttgctgctgagcaacccgaactcaacgccggatttctctgtagatgata
gcgaaggcgtagcagaaactaacgaagatttttaa