LOCUS       BBa_K648125             2658 bp    DNA     linear       25-Nov-2024
DEFINITION  Or tester with cI repressor with slightly weak RBS
ACCESSION   BBa_K648125
VERSION     BBa_K648125.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP protein
     CDS             1..720
                     /label=BBa_E0040
     misc_feature    733..736
                     /label=conserved
     RBS             729..740
                     /label=BBa_B0034
     CDS             749..830
                     /label=BBa_K648029
     misc_feature    843..846
                     /label=conserved
     RBS             839..850
                     /label=BBa_B0034
     CDS             857..1564
                     /label=mCherry
     misc_feature    1208..1208
                     /label=C->T (removing PstI site)
     misc_feature    1565..1573
                     /label=LVA
     misc_feature    857..1579
                     /label=BBa_J06505
     stem_loop       1599..1642
                     /label=stem_loop
     misc_feature    1588..1667
                     /label=BBa_B0010
     terminator      1588..1667
                     /label=BBa_B0010
     stem_loop       1683..1702
                     /label=T7 TE
     misc_feature    1709..1709
                     /label=stop
     polyA_site      1703..1716
                     /label=polya
     misc_feature    1676..1716
                     /label=BBa_B0012
     terminator      1676..1716
                     /label=BBa_B0012
     terminator      1588..1716
                     /label=BBa_B0015
     misc_feature    839..1716
                     /label=BBa_K648000
     promoter        1725..1855
                     /label=promoter
     misc_feature    1764..1781
                     /label=AraI1
     misc_feature    1785..1802
                     /label=AraI2
     promoter        1725..1854
                     /label=BBa_K206000
     RBS             1863..1877
                     /label=RBS-1\Strong
     RBS             1870..1874
                     /label=RBS
     misc_feature    1863..1877
                     /label=BBa_B0030
     RBS             1863..1877
                     /label=BBa_B0030
     CDS             1887..2594
                     /label=cI lambda
     misc_feature    2595..2627
                     /label=LVA
     misc_feature    2634..2658
                     /label=Help:Barcodes
     CDS             1884..2658
                     /label=BBa_C0051
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagagaa agaggagaaa tactagagac gttaaatcta tcaccgcaag ggataaatat
      781 ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa
      841 agaggagaaa tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      901 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      961 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1021 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     1081 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1141 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1201 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1261 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1321 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1381 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1441 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     1501 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1561 caagttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1621 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1681 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca
     1741 cggcgtcaca ctttgctatg ccatagcaag atagtccata agattagcgg atcctacctg
     1801 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag
     1861 agattaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc
     1921 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat
     1981 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta
     2041 atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta
     2101 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta
     2161 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag
     2221 ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca
     2281 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg
     2341 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc
     2401 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca
     2461 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa
     2521 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg
     2581 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
     2641 agtgctagtg tagatcgc
//