##gff-version 3
##sequence-region BBa_K648125 1 2658
BBa_K648125	.	ribosome_entry_site	1863	1877	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K648125	.	engineered_region	1863	1877	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K648125	.	ribosome_entry_site	1870	1874	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K648125	.	ribosome_entry_site	1863	1877	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K648125	.	CDS	749	830	.	+	0	ID=BBa_K648029;Name=BBa_K648029
BBa_K648125	.	promoter	1725	1854	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K648125	.	promoter	1725	1855	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K648125	.	operator	1764	1781	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K648125	.	operator	1785	1802	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K648125	.	engineered_region	839	1716	.	+	0	ID=BBa_K648000;Name=BBa_K648000
BBa_K648125	.	engineered_region	857	1579	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K648000
BBa_K648125	.	sequence_alteration	1208	1208	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K648125	.	CDS	857	1564	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K648125	.	tag	1565	1573	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K648125	.	terminator	1588	1716	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K648000
BBa_K648125	.	terminator	1676	1716	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K648125	.	stem_loop	1683	1702	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K648125	.	engineered_region	1676	1716	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K648125	.	stop_codon	1709	1709	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K648125	.	polyA_site	1703	1716	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K648125	.	terminator	1588	1667	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K648125	.	engineered_region	1588	1667	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K648125	.	stem_loop	1599	1642	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K648125	.	ribosome_entry_site	839	850	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K648000
BBa_K648125	.	conserved_region	843	846	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K648125	.	CDS	1884	2658	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K648125	.	engineered_tag	2634	2658	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K648125	.	CDS	1887	2594	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K648125	.	tag	2595	2627	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K648125	.	CDS	1	720	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K648125	.	CDS	1	720	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K648125	.	ribosome_entry_site	729	740	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K648125	.	conserved_region	733	736	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K648125
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa
cagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaact
acaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaaga
agatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagaaagaggagaaatactagagacgttaaatctatcaccgcaagggataaatatctaacaccgt
gcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagg
gcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacga
gttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggc
cccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgaca
tccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggt
gaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctcc
gacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctga
agggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaa
gaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatc
gtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttg
cacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaac
tctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatgagcacaaaaa
agaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttgg
cttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgca
ttaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagag
aaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgt
tcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaa
gccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctg
acggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatga
gtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatccca
tgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc