##gff-version 3
##sequence-region BBa_K648129 1 1910
BBa_K648129	.	CDS	1136	1910	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K648129	.	engineered_tag	1886	1910	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K648129	.	CDS	1139	1846	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K648129	.	tag	1847	1879	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K648129	.	CDS	1	82	.	+	0	ID=BBa_K648029;Name=BBa_K648029
BBa_K648129	.	ribosome_entry_site	1115	1129	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K648129	.	ribosome_entry_site	1115	1129	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K648129	.	engineered_region	1115	1129	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K648129	.	ribosome_entry_site	1122	1126	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K648129	.	engineered_region	91	968	.	+	0	ID=BBa_K648000;Name=BBa_K648000
BBa_K648129	.	ribosome_entry_site	91	102	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K648000
BBa_K648129	.	conserved_region	95	98	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K648129	.	engineered_region	109	831	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K648000
BBa_K648129	.	CDS	109	816	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K648129	.	sequence_alteration	460	460	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K648129	.	tag	817	825	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K648129	.	terminator	840	968	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K648000
BBa_K648129	.	terminator	840	919	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K648129	.	stem_loop	851	894	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K648129	.	engineered_region	840	919	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K648129	.	terminator	928	968	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K648129	.	stem_loop	935	954	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K648129	.	stop_codon	961	961	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K648129	.	polyA_site	955	968	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K648129	.	engineered_region	928	968	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K648129	.	promoter	977	1106	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K648129	.	promoter	977	1107	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K648129	.	operator	1016	1033	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K648129	.	operator	1037	1054	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
>BBa_K648129
acgttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggt
tgctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcat
gcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctac
gagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagt
tcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggctt
caagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgag
ttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctggg
aggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacgg
cggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaac
atcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactcca
ccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagt
ccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
tactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacg
tcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatg
gggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattc
tcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagcc
gtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaaccttt
accaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggta
attccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgt
tgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcag
gtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcg
ctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtg
ctagtgtagatcgc