LOCUS BBa_K648131 1907 bp DNA linear 22-Dec-2024 DEFINITION Pr of Or tester with cI repressor ACCESSION BBa_K648131 VERSION BBa_K648131.1 FEATURES Location/Qualifiers CDS 1..82 /label=BBa_K648029 misc_feature 95..98 /label=conserved RBS 91..102 /label=BBa_B0034 CDS 109..816 /label=mCherry misc_feature 460..460 /label=C->T (removing PstI site) misc_feature 817..825 /label=LVA misc_feature 109..831 /label=BBa_J06505 stem_loop 851..894 /label=stem_loop misc_feature 840..919 /label=BBa_B0010 terminator 840..919 /label=BBa_B0010 stem_loop 935..954 /label=T7 TE misc_feature 961..961 /label=stop polyA_site 955..968 /label=polya misc_feature 928..968 /label=BBa_B0012 terminator 928..968 /label=BBa_B0012 terminator 840..968 /label=BBa_B0015 misc_feature 91..968 /label=BBa_K648000 promoter 977..1107 /label=promoter misc_feature 1016..1033 /label=AraI1 misc_feature 1037..1054 /label=AraI2 promoter 977..1106 /label=BBa_K206000 misc_feature 1119..1122 /label=conserved RBS 1115..1126 /label=BBa_B0034 CDS 1136..1843 /label=cI lambda misc_feature 1844..1876 /label=LVA misc_feature 1883..1907 /label=Help:Barcodes CDS 1133..1907 /label=BBa_C0051 ORIGIN 1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac 61 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggtgagcaag 121 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 181 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 241 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 301 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 361 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 421 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc 481 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 541 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 601 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 661 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 721 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 781 cgccactcca ccggcggcat ggacgagctg tacaagttag tagcttaata atactagagc 841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 961 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca 1021 agatagtcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1081 tctccatacc gtttttttgg gctagctact agagaaagag gagaaatact agatgagcac 1141 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta 1201 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat 1261 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc 1321 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag 1381 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga 1441 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac 1501 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg 1561 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga 1621 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc 1681 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt 1741 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt 1801 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa 1861 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgc //