LOCUS       BBa_K649105             3618 bp    DNA     linear       22-Dec-2024
DEFINITION  PlsrA-gfp-PlsrR-lsrR
ACCESSION   BBa_K649105
VERSION     BBa_K649105.1  
FEATURES             Location/Qualifiers
     promoter        1..98
                     /label=PlsrA
     promoter        1..97
                     /label=BBa_K649100
     RBS             106..120
                     /label=RBS-1\Strong
     RBS             113..117
                     /label=RBS
     misc_feature    106..120
                     /label=BBa_B0030
     RBS             106..120
                     /label=BBa_B0030
     CDS             127..846
                     /label=GFP protein
     CDS             127..846
                     /label=BBa_E0040
     misc_feature    106..846
                     /label=BBa_E5500
     misc_feature    1..846
                     /label=BBa_K649104
     promoter        855..991
                     /label=lsrR promoter
     CDS             993..1946
                     /label=LsrR
     misc_feature    855..3618
                     /label=BBa_K649101
ORIGIN
        1 attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga acaaattaaa
       61 agcagaaata catttgttca aaactcacct gcaaaactac tagagattaa agaggagaaa
      121 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      181 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      241 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      301 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      361 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      421 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      481 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      541 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      601 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      661 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      721 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      841 taataatact agagttatgc tattttgatt ttcacggttg cgtttgttca tgctcgtaga
      901 gtcaaactgt ggttgccatc acagatataa atgagcaaga actgaacaat tgcattaaag
      961 atttaaatat gttcaaagtg aagaatgaat tatgacaatc aacgattcgg caatttcaga
     1021 acagggaatg tgtgaagaag aacaggtcgc gcggatcgcg tggttttact atcacgacgg
     1081 gctgacccag agcgagatca gcgatcgtct cggcctgaca cgtttgaaag tgtcgcgatt
     1141 gctggagaaa gggcatcagt ccggcattat tcgcgtacag attaattctc gctttgaagg
     1201 ctgtctggaa tatgaaactc aattacgtcg tcagttttcg ctgcaacatg tccgggtgat
     1261 ccctgggctt gcggatgctg atgtcggtgg gcgactgggg ataggcgcgg cgcatatgtt
     1321 gatgagttta cttcaaccac aacagatgct ggcgattggt tttggcgagg caaccatgaa
     1381 tacgctgcaa cgcttaagtg gttttatttc gtcacagcaa attcgcctgg tcacgctctc
     1441 cggtggcgtc ggttcttata tgacgggaat cgggcagctt aacgcggcgt gcagtgtgaa
     1501 tattattccg gctccgttgc gggcatcctc cgctgacatt gcccgtacgc taaaaaatga
     1561 aaattgcgtc aaagatgttc tgttagccgc gcaagcagcg gatgtggcga ttgtcggcat
     1621 tggtgctgtg agtcaacagg acgatgcgac aatcattcgc tccggttata tcagccaggg
     1681 cgaacagtta atgattgggc cgaaaagggg cggttggcga cattttaggc tacttttttg
     1741 atgcaaaagg tgacgttgtc acgaatatca aaatacataa cgaactgatt ggcttacctt
     1801 taagcgcgct gaagaccata cccgtccggg ttggcgtggc agggggagaa aataaagccg
     1861 aagcaattgc cgctgcaatg aaaggcggtt atatcaacgc actggttacc gatcaggaca
     1921 cagcagcggc gattttacgt agttaaattt gcatgacctt attaccggga atgatggaga
     1981 acgtatagag gaacagacag aacgcataag ccgaggataa tctaatggct cgactcttta
     2041 ccctttcaga atcaaagtac tacctgatgg cgctggatgc aggcaccgga agtattcggg
     2101 ctgtgatatt cgacctggaa ggcaatcaaa tagcagtggg acaggcggag tggcggcatc
     2161 tggcagtacc ggacgttcct ggttctatgg aatttgatct caacaaaaac tggcaactgg
     2221 cgtgtgagtg tatgcgccag gcgctgcaca acgccggcat agccccggag tatatcgctg
     2281 ccgtttcggc atgttcgatg cgtgaaggca ttgttttata taataatgaa ggagccccga
     2341 tctgggcctg cgccaatgtg gatgccagag cggcacgcga agttagcgaa cttaaagaac
     2401 tgcacaacaa tacctttgaa aacgaagttt atcgcgcgac cggacaaaca ctggctttaa
     2461 gtgccatccc cagattactt tggctggcgc accatcgttc cgatatttac cgtcaggcat
     2521 caaccatcac catgatcagc gactggctgg cctatatgct cagcggcgaa ctggcggtgg
     2581 atccctctaa cgctggcacc acgggacttc ttgatctaac cacccgtgac tggaaacctg
     2641 cattgctgga tatggctggc ctacgtgccg atattctttc tcctgtcaaa gaaaccggca
     2701 cattgctggg cgtggtaagt tcacaagcgg cggaactctg cggtctgaag gcgggcactc
     2761 cggtggtcgt tggaggaggc gacgtgcagc ttggttgcct tgggttaggc gttgtgcgtc
     2821 cggcacaaac cgcggttctt ggcggcacat tctggcagca agttgtaaat ttagccgcgc
     2881 cggtgacaga cccagaaatg aacgtgcgcg ttaatcctca tgttattcct ggcatggtac
     2941 aagctgaatc tataagcttt tttaccggac tcaccatgcg ctggttccgc gatgctttct
     3001 gtgccgaaga aaaactgatt gccgaacgtt taggcatcga cacctatacg ctgctggaag
     3061 agatggccag tcgggtgccg cctgggtcgt ggggcgtaat gccgatcttc tccgacagaa
     3121 tgcgctttaa aacctggtat cacgctgcgc cttcctttat taacttgtcc attgacccgg
     3181 ataaatgtaa caaagcgaca ttgttccgtg cgctggaaga aaatgcggcg attgtatcag
     3241 cgtgtaactt gcagcaaatt gctgatttct cgaatattca tccttcatcg ttagtctttg
     3301 caggcggagg ttcaaaaggg aaattatgga gtcaaattct cgctgatgtc tcgggattac
     3361 ccgtcaatat tccggtggtc aaagaagcca ctgcattagg atgtgccatt gcagctggcg
     3421 tcggtgccgg aattttttca tcaatggcag aaaccggaga acgcctggtt cgctgggaac
     3481 ggacgcacac accagacccg gaaaagcatg aactttatca ggattcacgc gataagtggc
     3541 aggcagttta tcaggatcag ctggggctgg ttgatcatgg actgacgacg tcgttatgga
     3601 aagcgccatg ggttatag
//