LOCUS       BBa_K649106             3557 bp    DNA     linear       16-Feb-2025
DEFINITION  PlsrA-gfp-PlacIQ-lsrR-lsrK
ACCESSION   BBa_K649106
VERSION     BBa_K649106.1  
FEATURES             Location/Qualifiers
     promoter        1..98
                     /label=PlsrA
     promoter        1..97
                     /label=BBa_K649100
     RBS             106..120
                     /label=RBS-1\Strong
     RBS             113..117
                     /label=RBS
     misc_feature    106..120
                     /label=BBa_B0030
     RBS             106..120
                     /label=BBa_B0030
     CDS             127..846
                     /label=GFP protein
     CDS             127..846
                     /label=BBa_E0040
     misc_feature    106..846
                     /label=BBa_E5500
     misc_feature    1..846
                     /label=BBa_K649104
     misc_feature    855..891
                     /label=P(Lac) IQ
     promoter        857..862
                     /label=-35
     promoter        880..885
                     /label=-10
     misc_feature    855..891
                     /label=BBa_I14032
     CDS             932..1886
                     /label=LsrR
     CDS             1965..3557
                     /label=LsrK
     misc_feature    911..3557
                     /label=BBa_K649102
     misc_feature    855..3557
                     /label=BBa_K649107
ORIGIN
        1 attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga acaaattaaa
       61 agcagaaata catttgttca aaactcacct gcaaaactac tagagattaa agaggagaaa
      121 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      181 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      241 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      301 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      361 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      421 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      481 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      541 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      601 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      661 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      721 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      841 taataatact agagtggtgc aaaacctttc gcggtatggc atgatagcgc ctactagacg
      901 gccgctacta gatcaaagtg aagaatgaat tatgacaatc aacgattcgg caatttcaga
      961 acaggaaatg tgtgaagaag aacaggtcgc gcggatcgcg tggttttact atcacgacgg
     1021 gctgacccag agcgagatca gcgatcgtct cggcctgaca cgtttgaaag tgtcgcgatt
     1081 gctggagaaa gggcatcagt ccggcattat tcgcgtacag attaattctc gctttgaagg
     1141 ctgtctggaa tatgaaactc aattacgtcg tcagttttcg ctgcaacatg tccgggtgat
     1201 ccctgggctt gcggatgctg atgtcggtgg gcgactgggg ataggcgcgg cgcatatgtt
     1261 gatgagttta cttcaaccac aacagatgct ggcgattggt tttggcgagg caaccatgaa
     1321 tacgctgcaa cgcttaagtg gttttatttc gtcacagcaa attcgcctgg tcacgctctc
     1381 cggtggcgtc ggttcttata tgacgggaat cgggcagctt aacgcggcgt gcagtgtgaa
     1441 tattattccg gctccgttgc gggcatcctc cgctgacatt gcccgtacgc taaaaaatga
     1501 aaattgcgtc aaagatgttc tgttagccgc gcaagcagcg gatgtggcga ttgtcggcat
     1561 tggtgctgtg agtcaacagg acgatgcgac aatcattcgc tccggttata tcagccaggg
     1621 cgaacagtta atgattgggc cgaaaagggg cggttggcga cattttaggc tacttttttg
     1681 atgcaaaagg tgacgttgtc acgaatatca aaatacataa cgaactgatt ggcttacctt
     1741 taagcgcgct gaagaccata cccgtccggg ttggcgtggc agggggagaa aataaagccg
     1801 aagcaattgc cgctgcaatg aaaggcggtt atatcaacgc actggttacc gatcaggaca
     1861 cagcagcggc gattttacgt agttaaattt gcatgacctt attaccggga atgatggaga
     1921 acgtatagag gaacagacag aacgcataag ccgaggataa tctaatggct cgactcttta
     1981 ccctttcaga atcaaagtac tacctgatgg cgctggatgc aggcaccgga agtattcggg
     2041 ctgtgatatt cgacctggaa ggcaatcaaa tagcagtggg acaggcggag tggcggcatc
     2101 tggcagtacc ggacgttcct ggttctatgg aatttgatct caacaaaaac tggcaactgg
     2161 cgtgtgagtg tatgcgccag gcgctgcaca acgccggcat agccccggag tatatcgctg
     2221 ccgtttcggc atgttcgatg cgtgaaggca ttgttttata taataatgaa ggagccccga
     2281 tctgggcctg cgccaatgtg gatgccagag cggcacgcga agttagcgaa cttaaagaac
     2341 tgcacaacaa tacctttgaa aacgaagttt atcgcgcgac cggacaaaca ctggctttaa
     2401 gtgccatccc cagattactt tggctggcgc accatcgttc cgatatttac cgtcaggcat
     2461 caaccatcac catgatcagc gactggctgg cctatatgct cagcggcgaa ctggcggtgg
     2521 atccctctaa cgctggcacc acgggacttc ttgatctaac cacccgtgac tggaaacctg
     2581 cattgctgga tatggctggc ctacgtgccg atattctttc tcctgtcaaa gaaaccggca
     2641 cattgctggg cgtggtaagt tcacaagcgg cggaactctg cggtctgaag gcgggcactc
     2701 cggtggtcgt tggaggaggc gacgtgcagc ttggttgcct tgggttaggc gttgtgcgtc
     2761 cggcacaaac cgcggttctt ggcggcacat tctggcagca agttgtaaat ttagccgcgc
     2821 cggtgacaga cccagaaatg aacgtgcgcg ttaatcctca tgttattcct ggcatggtac
     2881 aagctgaatc tataagcttt tttaccggac tcaccatgcg ctggttccgc gatgctttct
     2941 gtgccgaaga aaaactgatt gccgaacgtt taggcatcga cacctatacg ctgctggaag
     3001 agatggccag tcgggtgccg cctgggtcgt ggggcgtaat gccgatcttc tccgacagaa
     3061 tgcgctttaa aacctggtat cacgctgcgc cttcctttat taacttgtcc attgacccgg
     3121 ataaatgtaa caaagcgaca ttgttccgtg cgctggaaga aaatgcggcg attgtatcag
     3181 cgtgtaactt gcagcaaatt gctgatttct cgaatattca tccttcatcg ttagtctttg
     3241 caggcggagg ttcaaaaggg aaattatgga gtcaaattct cgctgatgtc tcgggattac
     3301 ccgtcaatat tccggtggtc aaagaagcca ctgcattagg atgtgccatt gcagctggcg
     3361 tcggtgccgg aattttttca tcaatggcag aaaccggaga acgcctggtt cgctgggaac
     3421 ggacgcacac accagacccg gaaaagcatg aactttatca ggattcacgc gataagtggc
     3481 aggcagttta tcaggatcag ctggggctgg ttgatcatgg actgacgacg tcgttatgga
     3541 aagcgcctgg gttatag
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