LOCUS       BBa_K651000             3061 bp    DNA     linear       07-Dec-2025
DEFINITION  RecA promoter with RFP coding device
ACCESSION   BBa_K651000
VERSION     BBa_K651000.1  
FEATURES             Location/Qualifiers
     CDS             193..867
                     /label=mrfp1
     misc_feature    874..898
                     /label=Help:Barcodes
     CDS             193..873
                     /label=BBa_E1010
     promoter        1..186
                     /label=BBa_J22126
     CDS             193..867
                     /label=mrfp1
     misc_feature    874..898
                     /label=Help:Barcodes
     CDS             193..898
                     /label=BBa_E1010
     misc_feature    1..898
                     /label=BBa_S03593
     CDS             905..2192
                     /label=BBa_J22101
     stem_loop       2212..2255
                     /label=stem_loop
     misc_feature    2201..2280
                     /label=BBa_B0010
     terminator      2201..2280
                     /label=BBa_B0010
     stem_loop       2296..2315
                     /label=T7 TE
     misc_feature    2322..2322
                     /label=stop
     polyA_site      2316..2329
                     /label=polya
     misc_feature    2289..2329
                     /label=BBa_B0012
     terminator      2289..2329
                     /label=BBa_B0012
     misc_feature    905..2329
                     /label=BBa_S03585
     misc_feature    1..2329
                     /label=BBa_J22146
     misc_feature    1..2329
                     /label=BBa_J22151
     misc_feature    2342..2345
                     /label=conserved
     RBS             2338..2349
                     /label=BBa_B0034
     CDS             2356..3030
                     /label=mrfp1
     misc_feature    3037..3061
                     /label=Help:Barcodes
     CDS             2356..3036
                     /label=BBa_E1010
ORIGIN
        1 aacaatttct acaaaacact tgatactgta tgagcataca gtataattgc ttcaacagaa
       61 catattgact atccggtatt acccggcatg acaggagtaa aaatggctat cgacgaaaac
      121 aaacagaaag cgttggcggc agcactgggc cagattgaga aacaatttgg taaaggctcc
      181 atcatgtact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt
      241 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
      301 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
      361 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
      421 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
      481 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
      541 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
      601 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
      661 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
      721 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
      781 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
      841 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta
      901 ctagatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa
      961 acttttggat gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc
     1021 cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta
     1081 tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg
     1141 acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta gtgatgtttg
     1201 cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta gtaggatcga
     1261 ttgttggtgg tatttatcta ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat
     1321 ttattgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg atgtttggct
     1381 gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc aataatcagt
     1441 ttgttttctg gctgggctct ggctgtgcac tcatcctcgc cgttttactc tttttcgcca
     1501 aaacggatgc gccctcttct gccacggttg ccaatgcggt aggtgccaac cattcggcat
     1561 ttagccttaa gctggcactg gaactgttca gacagccaaa actgtggttt ttgtcactgt
     1621 atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct aatttcttta
     1681 cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta acgacaatgg
     1741 gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat cgcatcggtg
     1801 ggaaaaacgc cctgctgctg gctggcacta ttatgtctgt acgtattatt ggctcatcgt
     1861 tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt gaagtaccgt
     1921 tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt ttttcagcga
     1981 cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt atgtctgtac
     2041 tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg ctgggtctgg
     2101 tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc ccgctttccc
     2161 tgctgcgtcg tcaggtgaat gaagtcgctt aatactagag ccaggcatca aataaaacga
     2221 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2281 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaaa
     2341 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa
     2401 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
     2461 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
     2521 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
     2581 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
     2641 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
     2701 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
     2761 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
     2821 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
     2881 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
     2941 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
     3001 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg
     3061 c
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