recA promoter was lifted via colony PCR from JM 109 strain of E.Coli with RFC 23 overhangs. RBS and RFP generator are existing biobricks from the registry. (B0034 and mRFP1 E1010)
This is a demo wetlab project performed by the 2011 NYC iGEM Software team to introduce them to basic practices of molecular biology, and to explore the steps and tools involved in putting together a simple genetic device. E.Coli recA promoter which had been colony PCR'd out of JM109 strain with RFC23 overhangs was combined with a ribosome binding site and mRFP1 E1010 coding device. This device is designed to produce RFP in presence of any external stimuli that can trigger the recA promoter. For purposes of NYC iGEM Software and our sister team NYC iGEM Wetware we focused on the device's ability to act as an ionizing radiation sensor.
Automated biobricking programs coupled with ability to look up genbank and other web based DNA databases speed up the ease and speed of compositing simple systems significantly.
Sung won Lim, Hannah Landes, Balvir Kunar
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
The Lac Y and mRFP gene under the RecA(SOS) promoter in plasmid.
The Lac Y and mRFP gene under the RecA(SOS) promoter has been inserted in plasmid pSB2K3 (part number BBa_I0500).
The Lac Y and mRFP gene under the RecA(SOS) promoter.
The Lac Y gene has been put under the RecA(SOS) promoter.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
The Rec A(SOS) promoter has been PCR amplified from the chromosomal DNA from MG1655(Ecoli).
The Lac Y gene has been PCR amplified from the chromosomal DNA of MG1655 (Escherechia coli)and has been inserted into plasmid pSB1A2( Part number BBa_B0015).
Conversion of the iGEM parts registry to SBOL2.1